CXCR5 Polyklonaler Antikörper

CXCR5 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28239-1-PBS

Synonyme

MDR 15, CXCR 5, CXC R5, CD185, Burkitt lymphoma receptor 1

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  WB analysis using 28239-1-AP (same clone as 28239-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28239-1-AP (CXCR5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28239-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 28239-1-AP (same clone as 28239-1-PBS)

  WB result of CXCR5 antibody (28239-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CXCR5 transfected Jurkat cells. This data was developed using the same antibody clone with 28239-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 28239-1-AP (same clone as 28239-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28239-1-AP (CXCR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28239-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28239-1-PBS bindet in WB, IHC, Indirect ELISA CXCR5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CXCR5 fusion protein Ag28271
• Vollständiger Name: chemokine (C-X-C motif) receptor 5
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: NM_001716
• Gene symbol: CXCR5
• Gene ID (NCBI): 643
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Initially discovered in 1992 as Burkitt's lymphoma receptor 1, CXCR5 (chemokine receptor 5, also known as CD185) is a seven-transmembrane G protein-coupled receptor (GPCR) protein (PMID: 32994482). CXCR5 is essential for naïve B-cell migration to and maturation in the lymph nodes and spleen, as well as for cluster of differentiation 4 T-cell (TFH and TCM) homing and interaction with B cells (PMID: 21471443). Moreover, CXCR5 is involved in the regulation of neural stem cells (NSC) and neuronal regeneration in the mouse brain, neuronal differentiation, neurogenesis, gliogenesis, and synaptogenesis (PMID: 37460877).