CXCR7 Polyklonaler Antikörper

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• KD/KO Validated

CXCR7 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20423-1-PBS

Synonyme

ACKR3, CXC R7, C-X-C chemokine receptor type 7, CMKOR1, Chemokine orphan receptor 1

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  WB analysis of Raji using 20423-1-AP (same clone as 20423-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 20423-1-AP (CXCR7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20423-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 20423-1-AP (same clone as 20423-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 20423-1-AP (CXCR7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20423-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 20423-1-AP (same clone as 20423-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20423-1-AP (CXCR7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20423-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 20423-1-AP (same clone as 20423-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20423-1-AP (CXCR7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20423-1-PBS in a different storage buffer formulation.

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  IP experiment of HUVEC using 20423-1-AP (same clone as 20423-1-PBS)

  IP result of anti-CXCR7 (IP:20423-1-AP, 3ug; Detection:20423-1-AP 1:500) with HUVEC cells lysate 1400ug. This data was developed using the same antibody clone with 20423-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 20423-1-AP (same clone as 20423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20423-1-AP (CXCR7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20423-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 20423-1-AP (same clone as 20423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20423-1-AP (CXCR7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20423-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20423-1-PBS bindet in WB, IHC, IP, Indirect ELISA CXCR7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CXCR7 fusion protein Ag14247
• Vollständiger Name: chemokine (C-X-C motif) receptor 7
• Berechnetes Molekulargewicht: 362 aa, 41 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC036661
• Gene symbol: CXCR7
• Gene ID (NCBI): 57007
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CXCR7 (C-X-C chemokine receptor type 7), also known as RDC1, is a member of the G-protein coupled receptor family. CXCR7 can bind the chemokines CXCL11 and CXCL12 with high affinity, and it also acts as coreceptor with CXCR4 for a restricted number of HIV isolates. Expression of CXCR7 has been associated with cardiac development as well as with tumor growth and progression. This antibody recognizes endogenous CXCR7, which has an experimentally determined molecular weight of 50 kDa (PMID: 20197403; 20388803).