CFAP47 Polyklonaler Antikörper

CFAP47 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 20837-1-AP

Synonyme

CXorf22, CHDC2, Cilia- and flagella-associated protein 47, CXorf30, CXorf59

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  WB analysis of mouse lung using 20837-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20837-1-AP (CXorf22 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 20837-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20837-1-AP (CXorf22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 20837-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20837-1-AP (CXorf22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 20837-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 20837-1-AP (CXorf22 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 20837-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 20837-1-AP (CXorf22 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 20837-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20837-1-AP (CXorf22 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 20837-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20837-1-AP (CXorf22 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse kidney using 20837-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using CFAP47 antibody (20837-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Lungengewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausnierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

20837-1-AP bindet in WB, IHC, IF-P, CoIP, ELISA CFAP47 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CFAP47 fusion protein Ag14910
• Vollständiger Name: chromosome X open reading frame 22
• Berechnetes Molekulargewicht: 976 aa, 110 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC027936
• Gene symbol: CFAP47/CXorf22
• Gene ID (NCBI): 170063
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CFAP47 (Cilia- and flagella-associated protein 47), formerly known as CXorf22, CXorf59, is located on the human chromosome X and highly expressed in the testis and encodes a cilia- and flagella-associated protein. CFAP47 was expressed in primary cilia of human kidney tubules, and knockout (KO) mice exhibited vacuolation of tubular cells and tubular dilation, providing evidence that CFAP47 is a causative gene involved in cyst formation (PMID: 33472045, 38633811). CFAP47-mutated male mice are sterile, with decreased sperm motility and abnormal flagella morphology (PMID: 37424856).

Publikationen

Species	Application	Title
mouse,human	WB,IF,CoIP	Mol Hum Reprod; WDR87 interacts with CFAP47 protein in the middle piece of spermatozoa flagella to participate in sperm tail assembly; Authors - Hong-Qing Liao
human	IF	medRxiv; CFAP47 is a novel causative gene implicated in X-linked polycystic kidney disease; Authors - Takayasu Mori