KK-LC-1 Polyklonaler Antikörper

KK-LC-1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 25708-1-AP

Synonyme

Cancer/testis antigen 83, CT83, CXorf61, KK LC 1, KKLC1, KK-LC-1

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  WB analysis using 25708-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25708-1-AP (KK-LC-1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 25708-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25708-1-AP (KK-LC-1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 25708-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25708-1-AP (KK-LC-1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 25708-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25708-1-AP (KK-LC-1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 25708-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25708-1-AP (KK-LC-1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 3 publications below

Produktinformation

25708-1-AP bindet in WB, IHC, ELISA KK-LC-1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KK-LC-1 fusion protein Ag22619
• Vollständiger Name: chromosome X open reading frame 61
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: BC062223
• Gene symbol: KK-LC-1
• Gene ID (NCBI): 203413
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KK-LC-1 has been described as kita-kyushu lung cancer antigen 1, cancer/testis antigen 83. Therefore, KK-LC-1 is also known as CXorf61 and CT83. KK-LC-1 has been tested for association to diseases, such as adenocarcinoma and lung neoplasms. The molecular mass of KK-LC-1 is 13 kDa. Studies have shown that KK-LC-1 is an ideal target for immunotherapy of triple-negative breast cancer (TNBC) (PMID: 26327325).

Publikationen

Species	Application	Title
human	IHC	Int Immunopharmacol; Integrative analysis-based identification and validation of a prognostic immune cell infiltration-based model for patients with advanced gastric cancer.; Authors - Siwei Pan
mouse	WB,IHC	Nat Commun; KK-LC-1 as a therapeutic target to eliminate ALDH+ stem cells in triple negative breast cancer; Authors - Jiawen Bu
human	IHC	Cancer Immunol Immunother; Integrating bulk and single-cell sequencing data to construct a Scissor+ dendritic cells prognostic model for predicting prognosis and immune responses in ESCC; Authors - Maosheng Cheng