CYB5B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CYB5B Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 15469-1-AP

Synonyme

CYB5 M, CYB5B, CYB5M, CYPB5M, Cytochrome b5 type B, OMB5

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Galerie der Validierungsdaten

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  WB analysis using 15469-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15469-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:4000 incubated at 4 degree celsius over night.

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  WB analysis of human liver using 15469-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 15469-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human adrenal gland using 15469-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15469-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15469-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15469-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 15469-1-AP

  IP Result of anti-CYB5B (IP:15469-1-AP, 3ug; Detection:15469-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of human testis using 15469-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15469-1-AP (CYB5B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 15469-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15469-1-AP (CYB5B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse testis using 15469-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15469-1-AP (CYB5B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 15469-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15469-1-AP (CYB5B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15469-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15469-1-AP (CYB5B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Lebergewebe, humanes Nebennierengewebe, Mauslebergewebe, Mauslungengewebe, Rattenlebergewebe, Rattenlungengewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

15469-1-AP bindet in WB, IP, IHC, IF, ELISA CYB5B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYB5B fusion protein Ag7771
• Vollständiger Name: cytochrome b5 type B (outer mitochondrial membrane)
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 20-21 kDa
• GenBank-Zugangsnummer: BC004373
• Gene symbol: CYB5B
• Gene ID (NCBI): 80777
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYB5B(Cytochrome b5 type B) is also named as CYB5M, OMB5 and belongs to the cytochrome b5 family. It is a 21 kDa membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. This protein is expressed on the plasma membrane of lymphoma cells but not normal lymphocytes, reactive lymphocytes or bone marrow precursor cells(PMID:20100355). The full length protein has a propeptide with 11 amino acids.

Publikationen

Species	Application	Title
human	WB	Cell Chem Biol; Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190.; Authors - Paige Dickson ; KD Validated
human	IHC	Front Mol Biosci; Comprehensive Analysis of mTORC1 Signaling Pathway-Related Genes in the Prognosis of HNSCC and the Response to Chemotherapy and Immunotherapy.; Authors - Zhao Ding
human	WB	J Biol Chem; Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome; Authors - Hana Nůsková