CYB5R1 Polyklonaler Antikörper

CYB5R1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11807-1-PBS

Synonyme

b5R.1, cytochrome b5 reductase 1, EC:1.6.2.2, Humb5R2, NADH cytochrome b5 reductase 1

---

• 

  WB analysis of human placenta using 11807-1-AP (same clone as 11807-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11807-1-AP (CYB5R1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11807-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 11807-1-AP (same clone as 11807-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11807-1-AP (CYB5R1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11807-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 11807-1-AP (same clone as 11807-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11807-1-AP (CYB5R1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11807-1-PBS in a different storage buffer formulation.

• 

  IP experiment of human placenta using 11807-1-AP (same clone as 11807-1-PBS)

  IP result of anti-CYB5R1 (IP:11807-1-AP, 4ug; Detection:11807-1-AP 1:1000) with human placenta tissue lysate 1520ug. This data was developed using the same antibody clone with 11807-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 11807-1-AP (same clone as 11807-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11807-1-AP (CYB5R1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11807-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HaCaT using 11807-1-AP (same clone as 11807-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using CYB5R1 antibody (11807-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11807-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

11807-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA CYB5R1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYB5R1 fusion protein Ag2475
• Vollständiger Name: cytochrome b5 reductase 1
• Berechnetes Molekulargewicht: 305 aa, 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC018732
• Gene symbol: CYB5R1
• Gene ID (NCBI): 51706
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.