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NOX2 Polyklonaler Antikörper

NOX2 Polyklonal Antikörper für WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IHC, IF-P, IF-Fro, FC (Intra), CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(209)

Kat-Nr. : 19013-1-AP

Synonyme

CYBB, CGD, CGD91-phox, Cytochrome b-245 heavy chain, EC:1.-.-.-



Geprüfte Anwendungen

Erfolgreiche Detektion in WBJ774A.1 cells, Mausmilzgewebe, Raji-Zellen, Rattenmilzgewebe, RAW 264.7-Zellen
Erfolgreiche Detektion in IHChumanes Tonsillitisgewebe, Mausmilzgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-PMausmilzgewebe, humanes Tonsillitisgewebe
Erfolgreiche Detektion in IF-FroMaushirngewebe
Erfolgreiche Detektion in FC (Intra)RAW 264.7-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
Immunhistochemie (IHC)IHC : 1:500-1:2000
Immunfluoreszenz (IF)-PIF-P : 1:200-1:800
Immunfluoreszenz (IF)-FROIF-FRO : 1:200-1:800
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

19013-1-AP bindet in WB, IHC, IF-P, IF-Fro, FC (Intra), CoIP, ELISA NOX2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Hund, Kaninchen, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen NOX2 fusion protein Ag5536
Vollständiger Name cytochrome b-245, beta polypeptide
Berechnetes Molekulargewicht 577 aa, 65 kDa
Beobachtetes Molekulargewicht 55 kDa
GenBank-ZugangsnummerBC032720
Gene symbol NOX2
Gene ID (NCBI) 1536
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NOX2, also named as CYBB, CGD, 91-phox, gp91-1, gp91-phox, p22 phagocyte B-cytochrome, cytochrome b-245 and beta polypeptide, is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. This full length protein has three glycosylation sites. CYBB is found in human cardiomyocytes as multiple bands:the signal between 55 and 65 kDa is probably the unglycosylated protein, because the predicted molecular weight of unglycosylated CYBB protein is approximately 58 kDa(PMID: 17587483) to 65 kDa in phagocytes and the bands around 80 kDa probably represent glycosylated CYBB, as has also been shown in human umbilical vein endothelial cells(PMID:12610097). In other reports, it also can be detected a band of 91 kDa(PMID:19965781).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for NOX2 antibody 19013-1-APProtokoll herunterladen
IHC protocol for NOX2 antibody 19013-1-APProtokoll herunterladenl
IF protocol for NOX2 antibody 19013-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouse,humanWB

Cell Host Microbe

Zinc promotes microbial p-coumaric acid production that protects against cholestatic liver injury

Authors - Dongping Li
mouseWB

Circulation

Megakaryocytic Leukemia 1 (MKL1) Bridges Epigenetic Activation of NADPH Oxidase in Macrophages to Cardiac Ischemia-Reperfusion Injury.

Authors - Yu Liming
mouseWB,IHC

Cell Host Microbe

Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation

Authors - Zhenyu Tian
mouseWB

Brain Behav Immun

Adiponectin ameliorates traumatic brain injury-induced ferroptosis through AMPK- ACC1 signaling pathway

Authors - Yufeng Ge

Sci Adv

Sphingolipid biosynthesis is essential for metabolic rewiring during TH17 cell differentiation

Authors - Thiruvaimozhi Abimannan
humanWB,IF

Redox Biol

Silencing COX-2 blocks PDK1/TRAF4-induced AKT activation to inhibit fibrogenesis during skeletal muscle atrophy.

Authors - Hongtao Chen