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CYFIP2 Rekombinanter Antikörper

CYFIP2 Rekombinant Antikörper für IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240909E3

Kat-Nr. : 83845-3-PBS

Synonyme

CYFIP1/2, 240909E3, p53-inducible protein 121, p53 inducible protein 121, KIAA1168

Filter:
  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CYFIP2 Recombinant antibody (83845-3-RR)

    IF Staining of Jurkat using 83845-3-RR (same clone as 83845-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CYFIP2 antibody (83845-3-RR, Clone: 240909E3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83845-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using CYFIP2 Recombinant antibody (83845-3-RR)

    FC experiment of Jurkat using 83845-3-RR (same clone as 83845-3-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Cyfip2 Recombinant Antibody (83845-3-RR, Clone:240909E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83845-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83845-3-RR

    Affinity and Kinetic Characterization of 83845-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83845-3-RR against Human CYFIP2 were performed. The affinity constant is 4.35 nM.

Geprüfte Anwendungen

Produktinformation

83845-3-PBS bindet in IF/ICC, FC (Intra), ELISA CYFIP2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CYFIP2 fusion protein Ag8749
Vollständiger Name cytoplasmic FMR1 interacting protein 2
Berechnetes Molekulargewicht 148 kDa
GenBank-ZugangsnummerBC011762
Gene symbol CYFIP2
Gene ID (NCBI) 26999
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYFIP1/2 belongs to the CYFIP family and is involved in T-cell adhesion and p53-dependent induction of apoptosis. CYFIP1 contains 1,253 amino acids and shares 88% sequence identity with CYFIP2. CYFIP1 and CYFIP2 are members of a highly conserved protein family and share approximately 99% sequence identity with their mouse orthologs. CYFIP1 has been shown to interact with the small GTPase RAC1, which is implicated in development and maintenance of neuronal structures. Consistent with FMRP and RAC1 localization in dendritic fine structures, CYFIP1/2 are present in synaptosomal extracts. This antibody can recognize both CYFIP1 and CYFIP2.