CYP11B2 Polyklonaler Antikörper

CYP11B2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 20968-1-AP

Synonyme

CYP11BL, CYP11B, CPN2, Corticosterone 18-monooxygenase, CYP11B2, Aldosterone-synthesizing enzyme

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  WB analysis using 20968-1-AP

  pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 20968-1-AP (CYP11B2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 20968-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 20968-1-AP (CYP11B2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human adrenal gland using 20968-1-AP

  Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue slide using 20968-1-AP (CYP11B2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human adrenal gland using 20968-1-AP

  Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue slide using 20968-1-AP (CYP11B2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 20968-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 20968-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 20968-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 20968-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 20968-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 20968-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of SKOV-3 using 20968-1-AP

  Immunofluorescent analysis of SKOV-3 cells using 20968-1-AP (CYP11B2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen, Hausschwein-Nebennierengewebe
• Erfolgreiche Detektion in IHC: humanes Nebennierengewebe, humanes Leberzirrhosegewebe, humanes Nierengewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SKOV-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

20968-1-AP bindet in WB, IHC, IF/ICC, ELISA CYP11B2 und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cytochrome P450, family 11, subfamily B, polypeptide 2
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: NM_000498
• Gene symbol: CYP11B2
• Gene ID (NCBI): 1585
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYP11B2 (Cytochrome P450 11B2), also known as aldosterone synthase, is a member of the cytochrome P450 superfamily of enzymes. CYP11B2 can only catalyze 11-deoxycorticosterone oxidation to produce corticosterone and further hydroxylation to ALD. CYP11B2 is an enzyme required for cortisol biosynthesis and is specifically expressed in aldosterone-producing tissue of the adrenal cortex and is overexpressed in aldosterone-producing adenomas (APA).

Publikationen

Species	Application	Title
human	IHC	Nat Commun; Adrenal tropism of SARS-CoV-2 and adrenal findings in a post-mortem case series of patients with severe fatal COVID-19.; Authors - Tanja Paul
human	WB,IHC	Cell Death Dis; Aldosterone from endometrial glands is benefit for human decidualization.; Authors - Shu-Yun Li
mouse	IF	PeerJ; Adrenal SGLT1 or SGLT2 as predictors of atherosclerosis under chronic stress based on a computer algorithm; Authors - Jianyi Li
human	IHC	Eur J Nucl Med Mol Imaging; Total or partial adrenalectomy for aldosterone-producing adenoma: can 68Ga-Pentixafor PET/CT predict surgical outcomes; Authors - Wen-Cai Zheng