CYP11B2 Polyklonaler Antikörper

CYP11B2 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20968-1-PBS

Synonyme

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  WB analysis using 20968-1-AP (same clone as 20968-1-PBS)

  pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 20968-1-AP (CYP11B2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

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  WB analysis of SKOV-3 using 20968-1-AP (same clone as 20968-1-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 20968-1-AP (CYP11B2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

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  IHC staining of human adrenal gland using 20968-1-AP (same clone as 20968-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue slide using 20968-1-AP (CYP11B2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

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  IHC staining of human adrenal gland using 20968-1-AP (same clone as 20968-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue slide using 20968-1-AP (CYP11B2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 20968-1-AP (same clone as 20968-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 20968-1-AP (same clone as 20968-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 20968-1-AP (same clone as 20968-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human hepatocirrhosis using 20968-1-AP (same clone as 20968-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 20968-1-AP (same clone as 20968-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 20968-1-AP (same clone as 20968-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SKOV-3 using 20968-1-AP (same clone as 20968-1-PBS)

  Immunofluorescent analysis of SKOV-3 cells using 20968-1-AP (CYP11B2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20968-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CYP11B2 und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cytochrome P450, family 11, subfamily B, polypeptide 2
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: NM_000498
• Gene symbol: CYP11B2
• Gene ID (NCBI): 1585
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYP11B2 (Cytochrome P450 11B2), also known as aldosterone synthase, is a member of the cytochrome P450 superfamily of enzymes. CYP11B2 can only catalyze 11-deoxycorticosterone oxidation to produce corticosterone and further hydroxylation to ALD. CYP11B2 is an enzyme required for cortisol biosynthesis and is specifically expressed in aldosterone-producing tissue of the adrenal cortex and is overexpressed in aldosterone-producing adenomas (APA).