CYP17A1 Polyklonaler Antikörper

CYP17A1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(50)

Rezensionen (3)

Kat-Nr. : 14447-1-AP

Synonyme

17-alpha-hydroxyprogesterone aldolase, CPT7, CYP17, CYPXVII, Cytochrome P450 17A1

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  WB analysis using 14447-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14447-1-AP (CYP17A1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14447-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14447-1-AP (CYP17A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 14447-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14447-1-AP (CYP17A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 14447-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14447-1-AP (CYP17A1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human prostate cancer using 14447-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14447-1-AP (CYP17A1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human prostate cancer using 14447-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14447-1-AP (CYP17A1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human prostate cancer using 14447-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14447-1-AP (CYP17A1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 14447-1-AP

  Immunofluorescent analysis of HepG2 cells, using CYP17A1 antibody 14447-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HepG2 using 14447-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CYP17A1 Polyclonal antibody (14447-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 27 publications below
• IHC: See 15 publications below
• IF: See 15 publications below

Produktinformation

14447-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA CYP17A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYP17A1 fusion protein Ag5899
• Vollständiger Name: cytochrome P450, family 17, subfamily A, polypeptide 1
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 50-57 kDa
• GenBank-Zugangsnummer: BC062997
• Gene symbol: CYP17A1
• Gene ID (NCBI): 1586
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cytochrome P450 17A1 (CYP17A1) is a critical steroidogenic enzyme, essential for producing glucocorticoids and sex hormones (PMID: 33781841). The CYP17A1 gene is expressed in the adrenal cortex and the gonads but not in the placenta (PMID: 19403566). Although CYP17A1 has been shown to be a 50-kDa protein, the presence of a lower molecular mass(30 kDa) immunoreactive form in rat Leydig cells was previously shown and it is either a degradation product or a truncated form of the 50 kDa enzyme (PMID: 15761033). Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5) and CYP17A1 is an important target for inhibition in the treatment of prostate cancer (PMID: 27505042).

Publikationen

Species	Application	Title
mouse	WB	Part Fibre Toxicol; Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway.; Authors - Haibo Jin
pig	WB	Biol Res; Unraveling the impact of hyperleptinemia on female reproduction: insights from transgenic pig model; Authors - Muhammad Ameen Jamal
human	IHC	J Clin Endocrinol Metab; PMID: 25137424; Authors - Kaisa Huhtinen
mouse	IHC	J Cell Sci; Transgenic GATA-4 expression induces adrenocortical tumorigenesis in C57Bl/6 mice.; Authors - Chrusciel Marcin M
mouse	IHC	FASEB J; Hydroxysteroid (17β)-dehydrogenase 1-deficient female mice present with normal puberty onset but are severely subfertile due to a defect in luteinization and progesterone production.; Authors - Janne Hakkarainen
mouse	IF	Development; WNT signaling in pre-granulosa cells is required for ovarian folliculogenesis and female fertility.; Authors - Okiko Habara

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (04-26-2024)

The size of the protein that the antibody recognizes in samples is much smaller than expected.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse liver and mouse brain

CYP17A1 Antibody Western Blot validation (1:1000 dilution) in mouse liver and mouse brain (Cat no:14447-1-AP)

FH

Tom (Verified Customer) (08-25-2021)

2 non-specific bands appear on membrane, but the correct band can be distinguished.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500

CYP17A1 Antibody Western Blot validation (1:500 dilution) in (Cat no:14447-1-AP)

FH

Hailey (Verified Customer) (10-07-2019)

This antibody works very well for IF in mouse testis. We use Cyp17a1 as a marker for a lot of my experiments, and after comparing Cyp17a1 antibodies from many companies, this is our antibody of choice for IF. However for westerns, there is a pesky non-specific band that appears right below the correct band, and it is difficult to differentiate them unless you run the WB for a long time.

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse testis

CYP17A1 Antibody Western Blot,Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Mouse testis (Cat no:14447-1-AP)