CYP19A1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CYP19A1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 16554-1-AP

Synonyme

ARO, ARO1, Aromatase, CPV1, CYAR, CYP19, CYP19A1, CYPXIX, Cytochrome P 450AROM, Cytochrome P450 19A1, Estrogen synthase, P 450AROM

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Galerie der Validierungsdaten

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  WB analysis of A2780 using 16554-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 16554-1-AP (CYP19A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 16554-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16554-1-AP (CYP19A1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 16554-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16554-1-AP (CYP19A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16554-1-AP (CYP19A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16554-1-AP (CYP19A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16554-1-AP (CYP19A1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16554-1-AP (CYP19A1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16554-1-AP (CYP19A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16554-1-AP (CYP19A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16554-1-AP (CYP19A1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16554-1-AP (CYP19A1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 16554-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CYP19A1 antibody (16554-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 16554-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CYP19A1 antibody (16554-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human placenta using 16554-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CYP19A1 antibody (16554-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human placenta using 16554-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CYP19A1 antibody (16554-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A2780-Zellen, humanes Plazenta-Gewebe, Maus-Eierstockgewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Eierstockgewebe, humanes Gliomgewebe, humanes Hodengewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nierengewebe, humanes Skelettmuskelgewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, humanes Plazenta-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

16554-1-AP bindet in WB, IHC, IF, ELISA CYP19A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Gänse, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYP19A1 fusion protein Ag9765
• Vollständiger Name: cytochrome P450, family 19, subfamily A, polypeptide 1
• Berechnetes Molekulargewicht: 20 kDa, 25 kDa, 58 kDa
• Beobachtetes Molekulargewicht: 49-55 kDa
• GenBank-Zugangsnummer: BC022896
• Gene symbol: CYP19A1
• Gene ID (NCBI): 1588
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYP19A1(Cytochrome P450 19A1) is also named as ARO1(aromatase), CYAR, CYP19, estrogen synthase and belongs to the cytochrome P450 family. It is a terminal enzyme which transforms irreversibly androgens into estrogens and it is present in the endoplasmic reticulum of numerous tissues(PMID:16406261). The gene encodes a protein with the molecular weight between 46 kDa and 69 kDa(PMID:8129748) and the protein can be glycosylated, but the level of glycosylation does not seem to be essential for CYP19A1 activity(PMID:12606587). Brain aromatase may be neuroprotective by increasing the local estrogen levels in injured neurons so that genetic variation in the brain aromatase gene may modify the risk for AD(PMID:16767510).

Publikationen

Species	Application	Title
human	WB	Clin Transl Med; Treg deficiency-mediated TH 1 response causes human premature ovarian insufficiency through apoptosis and steroidogenesis dysfunction of granulosa cells.; Authors - Xue Jiao
mouse	WB	Sci Rep; Novel WT1 Missense Mutations in Han Chinese Women with Premature Ovarian Failure.; Authors - Huidan Wang
mouse, rat	WB	Sci Rep; Estrogen Secreted by Mesenchymal Stem Cells Necessarily Determines Their Feasibility of Therapeutical Application.; Authors - Jiansha Li ; KD Validated
human	WB,IHC	Fertil Steril; Elevated maternal androgen is associated with dysfunctional placenta and lipid disorder in newborns of mothers with polycystic ovary syndrome.; Authors - Miao Sun
rat	WB	Environ Pollut; Hormonal changes and folliculogenesis in female offspring of rats exposed to cadmium during gestation and lactation.; Authors - Zhiliang Li
mouse	WB	Endocrinology; The Immp2l Mutation Causes Ovarian Aging Through ROS-Wnt/β-Catenin-Estrogen Pathway: Preventive Effect of Melatonin.; Authors - Qing He