CYP19A1 Polyklonaler Antikörper

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• KD/KO Validated

CYP19A1 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16554-1-PBS

Synonyme

ARO, ARO1, Aromatase, CPV1, CYAR

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  WB analysis of A2780 using 16554-1-AP (same clone as 16554-1-PBS)

  A2780 cells were subjected to SDS PAGE followed by western blot with 16554-1-AP (CYP19A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 16554-1-AP (same clone as 16554-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16554-1-AP (CYP19A1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.

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  WB analysis using 16554-1-AP (same clone as 16554-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16554-1-AP (CYP19A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 16554-1-AP (same clone as 16554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16554-1-AP (CYP19A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 16554-1-AP (same clone as 16554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16554-1-AP (CYP19A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 16554-1-AP (same clone as 16554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16554-1-AP (CYP19A1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 16554-1-AP (same clone as 16554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16554-1-AP (CYP19A1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 16554-1-AP (same clone as 16554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16554-1-AP (CYP19A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 16554-1-AP (same clone as 16554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16554-1-AP (CYP19A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 16554-1-AP (same clone as 16554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16554-1-AP (CYP19A1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 16554-1-AP (same clone as 16554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16554-1-AP (CYP19A1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 16554-1-AP (same clone as 16554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 16554-1-AP (same clone as 16554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 16554-1-AP (same clone as 16554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 16554-1-AP (same clone as 16554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 16554-1-AP (same clone as 16554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 16554-1-AP (same clone as 16554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 16554-1-AP (same clone as 16554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary using 16554-1-AP (same clone as 16554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary using 16554-1-AP (same clone as 16554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 16554-1-AP (same clone as 16554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 16554-1-AP (same clone as 16554-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CYP19A1 antibody (16554-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 16554-1-AP (same clone as 16554-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CYP19A1 antibody (16554-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 16554-1-AP (same clone as 16554-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CYP19A1 antibody (16554-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16554-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA CYP19A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYP19A1 fusion protein Ag9765
• Vollständiger Name: cytochrome P450, family 19, subfamily A, polypeptide 1
• Berechnetes Molekulargewicht: 20 kDa, 25 kDa, 58 kDa
• Beobachtetes Molekulargewicht: 49-55 kDa
• GenBank-Zugangsnummer: BC022896
• Gene symbol: CYP19A1
• Gene ID (NCBI): 1588
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYP19A1(Cytochrome P450 19A1) is also named as ARO1(aromatase), CYAR, CYP19, estrogen synthase and belongs to the cytochrome P450 family. It is a terminal enzyme which transforms irreversibly androgens into estrogens and it is present in the endoplasmic reticulum of numerous tissues(PMID:16406261). The gene encodes a protein with the molecular weight between 46 kDa and 69 kDa(PMID:8129748) and the protein can be glycosylated, but the level of glycosylation does not seem to be essential for CYP19A1 activity(PMID:12606587). Brain aromatase may be neuroprotective by increasing the local estrogen levels in injured neurons so that genetic variation in the brain aromatase gene may modify the risk for AD(PMID:16767510).