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CYP1B1 Polyklonaler Antikörper

CYP1B1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(26)

Kat-Nr. : 18505-1-AP

Synonyme

Hydroperoxy icosatetraenoate dehydratase, GLC3A, EC:4.2.1.152, EC:1.14.14.1, CYPIB1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBPC-3-Zellen, MCF-7-Zellen, Mausherzgewebe, Rattenhirngewebe
Erfolgreiche Detektion in IHChumanes Kolonkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunhistochemie (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

18505-1-AP bindet in WB, IHC, IF, ELISA CYP1B1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Affe, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CYP1B1 fusion protein Ag13357
Vollständiger Name cytochrome P450, family 1, subfamily B, polypeptide 1
Berechnetes Molekulargewicht 61 kDa
Beobachtetes Molekulargewicht 52 kDa
GenBank-ZugangsnummerBC012049
Gene symbol CYP1B1
Gene ID (NCBI) 1545
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cytochrome P450 CYP1B1 is a recently cloned dioxin-inducible form of the cytochrome P450 family of xenobiotic metabolizing enzymes. CYP1B1 was found to be expressed at a high frequency in a wide range of human cancers of different histogenetic types, including cancers of the breast, colon, lung, esophagus, skin, lymph node, brain, and testis. There was no detectable immunostaining for CYP1B1 in normal tissues.(PMID:9230218). CYP1B1 is a 60 kDa protein that it also can be detected a band of 51 kDa or 57 kDa through the western blot(PMID:9525272; PMID: 9525272).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for CYP1B1 antibody 18505-1-APProtokoll herunterladen
IHC protocol for CYP1B1 antibody 18505-1-APProtokoll herunterladenl
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB,IF

Cell Death Differ

The AKR1C1-CYP1B1-cAMP signaling axis controls tumorigenicity and ferroptosis susceptibility of extrahepatic cholangiocarcinoma

Authors - Chang Liu
mouseIF

NPJ Regen Med

Myeloid cell-associated aromatic amino acid metabolism facilitates CNS myelin regeneration

Authors - Jingwen Hu
humanWB

BMC Med

NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma

Authors - Yizhi Zhan
humanWB

PLoS Pathog

Reduced IRF4 expression promotes lytic phenotype in Type 2 EBV-infected B cells.

Authors - Jillian A Bristol
mouseWB

Front Pharmacol

Astilbin Activates the Reactive Oxidative Species/PPARγ Pathway to Suppress Effector CD4+ T Cell Activities via Direct Binding With Cytochrome P450 1B1.

Authors - Shizhen Ding
humanWB

J Transl Med

piRNA-14633 promotes cervical cancer cell malignancy in a METTL14-dependent m6A RNA methylation manner.

Authors - Qi Xie

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Hala (Verified Customer) (11-23-2021)

works very good

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: hepG2, hela cells