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CYP1B1 Polyklonaler Antikörper
CYP1B1 Polyklonal Antikörper für WB, IHC, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (1)
Anwendung
WB, IHC, IF, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 18505-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | PC-3-Zellen, MCF-7-Zellen, Mausherzgewebe, Rattenhirngewebe |
Erfolgreiche Detektion in IHC | humanes Kolonkarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunhistochemie (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 1 publications below |
WB | See 19 publications below |
IHC | See 3 publications below |
IF | See 5 publications below |
Produktinformation
18505-1-AP bindet in WB, IHC, IF, ELISA CYP1B1 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Affe, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | CYP1B1 fusion protein Ag13357 |
Vollständiger Name | cytochrome P450, family 1, subfamily B, polypeptide 1 |
Berechnetes Molekulargewicht | 61 kDa |
Beobachtetes Molekulargewicht | 52 kDa |
GenBank-Zugangsnummer | BC012049 |
Gene symbol | CYP1B1 |
Gene ID (NCBI) | 1545 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Cytochrome P450 CYP1B1 is a recently cloned dioxin-inducible form of the cytochrome P450 family of xenobiotic metabolizing enzymes. CYP1B1 was found to be expressed at a high frequency in a wide range of human cancers of different histogenetic types, including cancers of the breast, colon, lung, esophagus, skin, lymph node, brain, and testis. There was no detectable immunostaining for CYP1B1 in normal tissues.(PMID:9230218). CYP1B1 is a 60 kDa protein that it also can be detected a band of 51 kDa or 57 kDa through the western blot(PMID:9525272; PMID: 9525272).
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for CYP1B1 antibody 18505-1-AP | Protokoll herunterladen |
IHC protocol for CYP1B1 antibody 18505-1-AP | Protokoll herunterladenl |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Cell Death Differ The AKR1C1-CYP1B1-cAMP signaling axis controls tumorigenicity and ferroptosis susceptibility of extrahepatic cholangiocarcinoma | ||
NPJ Regen Med Myeloid cell-associated aromatic amino acid metabolism facilitates CNS myelin regeneration | ||
BMC Med NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma | ||
PLoS Pathog Reduced IRF4 expression promotes lytic phenotype in Type 2 EBV-infected B cells. | ||
Front Pharmacol Astilbin Activates the Reactive Oxidative Species/PPARγ Pathway to Suppress Effector CD4+ T Cell Activities via Direct Binding With Cytochrome P450 1B1. | ||
J Transl Med piRNA-14633 promotes cervical cancer cell malignancy in a METTL14-dependent m6A RNA methylation manner. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Hala (Verified Customer) (11-23-2021) | works very good
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