CYP1B1 Monoklonaler Antikörper

CYP1B1 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G8D4

Kat-Nr. : 67033-1-PBS

Synonyme

GLC3A, EC:4.2.1.152, EC:1.14.14.1, CYPIB1, CP1B

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  WB analysis using 67033-1-Ig (same clone as 67033-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67033-1-Ig (CYP1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 67033-1-Ig (same clone as 67033-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67033-1-Ig (CYP1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 67033-1-Ig (same clone as 67033-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67033-1-Ig (CYP1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 67033-1-Ig (same clone as 67033-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67033-1-Ig (CYP1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 67033-1-Ig (same clone as 67033-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67033-1-Ig (CYP1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67033-1-Ig (same clone as 67033-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 67033-1-Ig (same clone as 67033-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 67033-1-Ig (same clone as 67033-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67033-1-Ig (same clone as 67033-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 67033-1-Ig (same clone as 67033-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 67033-1-Ig (same clone as 67033-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67033-1-PBS bindet in WB, IHC, Indirect ELISA CYP1B1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CYP1B1 fusion protein Ag13380
• Vollständiger Name: cytochrome P450, family 1, subfamily B, polypeptide 1
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC012049
• Gene symbol: CYP1B1
• Gene ID (NCBI): 1545
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cytochrome P450 CYP1B1 is a recently cloned dioxin-inducible form of the cytochrome P450 family of xenobiotic metabolizing enzymes. CYP1B1 was found to be expressed at a high frequency in a wide range of human cancers of different histogenetic types, including cancers of the breast, colon, lung, esophagus, skin, lymph node, brain, and testis. There was no detectable immunostaining for CYP1B1 in normal tissues.(PMID:9230218). CYP1B1 is a 60 kDa protein that it also can be detected a band of 51 kDa or 57 kDa through the western blot(PMID:9525272; PMID: 9525272).