CYP27A1 Polyklonaler Antikörper

CYP27A1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14739-1-PBS

Synonyme

Cytochrome P450 27, Cytochrome P 450C27/25, CYP27, CTX, CP27

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  WB analysis of mouse liver using 14739-1-AP (same clone as 14739-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14739-1-AP (CYP27A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14739-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 14739-1-AP (same clone as 14739-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14739-1-AP (CYP27A1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14739-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 14739-1-AP (same clone as 14739-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14739-1-AP (CYP27A1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14739-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14739-1-AP (same clone as 14739-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14739-1-AP (CYP27A1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14739-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 14739-1-AP (same clone as 14739-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 14739-1-AP (CYP27A1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14739-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 14739-1-AP (same clone as 14739-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 14739-1-AP (CYP27A1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14739-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14739-1-PBS bindet in WB, IHC, Indirect ELISA CYP27A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYP27A1 fusion protein Ag6408
• Vollständiger Name: cytochrome P450, family 27, subfamily A, polypeptide 1
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 60-65 kDa
• GenBank-Zugangsnummer: BC051851
• Gene symbol: CYP27A1
• Gene ID (NCBI): 1593
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mitochondrial sterol 27-hydroxylase (CYP27A1) catalyzes oxidative cleavage of the sterol side chain in the bile acid biosynthetic pathway in the liver and 27-hydroxylation of cholesterol in most tissues(PMID:17088262). It belongs to the cytochrome P450 family. CYP27A1 catalyses an important sterol elimination pathway in the macrophage, and consequently may protect against atherosclerosis.