CYP27A1 Monoklonaler Antikörper

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• KD/KO Validated

CYP27A1 Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

3G3F7

Kat-Nr. : 67045-1-PBS

Synonyme

3G3F7, 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 26-hydroxylase, CP27, CTX, CYP27

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  WB analysis of Caco-2 using 67045-1-Ig (same clone as 67045-1-PBS)

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67045-1-Ig (same clone as 67045-1-PBS)

  WB result of CYP27A1 antibody (67045-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP27A1 transfected HepG2 cells. This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67045-1-Ig (same clone as 67045-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-29 using 67045-1-Ig (same clone as 67045-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67045-1-Ig (same clone as 67045-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-29 using 67045-1-Ig (same clone as 67045-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 67045-1-Ig (same clone as 67045-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67045-1-Ig (CYP27A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67045-1-Ig (CYP27A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CYP27A1 antibody (67045-1-Ig, Clone: 3G3F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CYP27A1 antibody (67045-1-Ig, Clone: 3G3F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67045-1-PBS bindet in WB, IHC, IF-P, ELISA CYP27A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CYP27A1 fusion protein Ag7746
• Vollständiger Name: cytochrome P450, family 27, subfamily A, polypeptide 1
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 54-60 kDa
• GenBank-Zugangsnummer: BC051851
• Gene symbol: CYP27A1
• Gene ID (NCBI): 1593
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.