CYP2E1-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CYP2E1-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19937-1-PBS

Synonyme

---

• 

  WB analysis using 19937-1-AP (same clone as 19937-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19937-1-AP (CYP2E1-Specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19937-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 19937-1-AP (same clone as 19937-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19937-1-AP (CYP2E1-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19937-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 19937-1-AP (same clone as 19937-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19937-1-AP (CYP2E1-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19937-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 19937-1-AP (same clone as 19937-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19937-1-AP (CYP2E1-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19937-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 19937-1-AP (same clone as 19937-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19937-1-AP (CYP2E1-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19937-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 19937-1-AP (same clone as 19937-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CYP2E1-Specific antibody (19937-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 19937-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 19937-1-AP (same clone as 19937-1-PBS)

  Immunofluorescent analysis of Hela cells, using CYP2E1 antibody 19937-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 19937-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

19937-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CYP2E1-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cytochrome P450, family 2, subfamily E, polypeptide 1
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 50-57 kDa
• GenBank-Zugangsnummer: NM_000773
• Gene symbol: CYP2E1
• Gene ID (NCBI): 1571
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYP2E1, also named as CYPIIE1, 4-nitrophenol 2-hydroxylase, P450-J and CYP2E, belongs to the cytochrome P450 family. CYP2E1 metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. It inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. CYP2E1 catalyze the reaction: 4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O. CYP2E1 can be detected as 50-57 kDa(full-length) and ~40 kDa(N-terminal signal peptide cleaved) (PMID: 21130154). The antibody is specific to CYP2E1.