CYP2E1-Specific Monoklonaler Antikörper

CYP2E1-Specific Monoklonal Antikörper für WB, IHC, IF-P, IF-Fro, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, Maus, Ratte

Anwendung

WB, IHC, IF-P, IF-Fro, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

CloneNo.

1F12G7

Kat-Nr. : 67263-1-Ig

Synonyme

CYP2E1, 1F12G7, 4-nitrophenol 2-hydroxylase, CPE1, CYP2E

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  WB analysis of HaCaT using 67263-1-Ig

  HaCaT cells were subjected to SDS PAGE followed by western blot with 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig liver using 67263-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 67263-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 67263-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 67263-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 67263-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67263-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67263-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 67263-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CYP2E1-Specific antibody (67263-1-Ig, Clone: 1F12G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 67263-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse liver tissue using 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Lebergewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mauslebergewebe
• Erfolgreiche Detektion in IF-Fro: Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:400-1:1600
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 2 publications below

Produktinformation

67263-1-Ig bindet in WB, IHC, IF-P, IF-Fro, ELISA CYP2E1-Specific und zeigt Reaktivität mit Hausschwein, Maus, Ratten

• Getestete Reaktivität: Hausschwein, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cytochrome P450, family 2, subfamily E, polypeptide 1
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 50-57 kDa
• GenBank-Zugangsnummer: NM_000773
• Gene symbol: CYP2E1
• Gene ID (NCBI): 1571
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYP2E1, also named as CYPIIE1, 4-nitrophenol 2-hydroxylase, P450-J and CYP2E, belongs to the cytochrome P450 family. CYP2E1 metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. It inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. CYP2E1 catalyze the reaction: 4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O. CYP2E1 can be detected as 50-57 kDa(full-length) and ~40 kDa(N-terminal signal peptide cleaved) (PMID: 21130154). The antibody is specific to CYP2E1.

Publikationen

Species	Application	Title
mouse	WB	Biomed Pharmacother; The effects of scoparone on alcohol and high-fat diet-induced liver injury revealed by RNA sequencing; Authors - Min Wei
mouse	WB,IHC	Front Pharmacol; Methyl 6-O-cinnamoyl-α-d-glucopyranoside Ameliorates Acute Liver Injury by Inhibiting Oxidative Stress Through the Activation of Nrf2 Signaling Pathway.; Authors - Qianqian Xu
mouse	WB	Med Sci Monit; Comparison of the Effects of Intraperitoneal Injection with Carbon Tetrachloride on Acute Liver Toxicity in Male and Female Kunming Mice; Authors - Huan Yang
mouse	WB	Nutrients; Daucosterol Alleviates Alcohol−Induced Hepatic Injury and Inflammation through P38/NF−κB/NLRP3 Inflammasome Pathway; Authors - Feng Zhang
mouse	IHC	J Ethnopharmacol; Protective effects of phenylethanol glycosides from Cistanche tubulosa against ALD through modulating gut microbiota homeostasis; Authors - Zhaoyao Qi
mouse	WB	Mol Nutr Food Res; Protective Effect and Mechanism of L-Theanine on Acute Alcoholic Liver Injury in Mice; Authors - Meng-Yuan Liu