CYP2E1-Specific Monoklonaler Antikörper

CYP2E1-Specific Monoklonal Antikörper für WB, IHC, IF-P, IF-Fro, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, Maus, Ratte

Anwendung

WB, IHC, IF-P, IF-Fro, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F12G7

Kat-Nr. : 67263-1-PBS

Synonyme

CYP2E1, 1F12G7, 4-nitrophenol 2-hydroxylase, CPE1, CYP2E

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  WB analysis of pig liver using 67263-1-Ig (same clone as 67263-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

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  WB analysis of HaCaT using 67263-1-Ig (same clone as 67263-1-PBS)

  HaCaT cells were subjected to SDS PAGE followed by western blot with 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 67263-1-Ig (same clone as 67263-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 67263-1-Ig (same clone as 67263-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67263-1-Ig (same clone as 67263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67263-1-Ig (same clone as 67263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67263-1-Ig (same clone as 67263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67263-1-Ig (same clone as 67263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 67263-1-Ig (same clone as 67263-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CYP2E1-Specific antibody (67263-1-Ig, Clone: 1F12G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 67263-1-Ig (same clone as 67263-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse liver tissue using 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67263-1-PBS bindet in WB, IHC, IF-P, IF-Fro, Indirect ELISA CYP2E1-Specific und zeigt Reaktivität mit Hausschwein, Maus, Ratten

• Getestete Reaktivität: Hausschwein, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cytochrome P450, family 2, subfamily E, polypeptide 1
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 50-57 kDa
• GenBank-Zugangsnummer: NM_000773
• Gene symbol: CYP2E1
• Gene ID (NCBI): 1571
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYP2E1, also named as CYPIIE1, 4-nitrophenol 2-hydroxylase, P450-J and CYP2E, belongs to the cytochrome P450 family. CYP2E1 metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. It inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. CYP2E1 catalyze the reaction: 4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O. CYP2E1 can be detected as 50-57 kDa(full-length) and ~40 kDa(N-terminal signal peptide cleaved) (PMID: 21130154). The antibody is specific to CYP2E1.