CYP2W1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CYP2W1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 12904-1-AP

Synonyme

CYPIIW1, Cytochrome P450 2W1, EC:1.14.14.-

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  WB analysis of HEK-293 using 12904-1-AP

  WB result of CYP2W1 antibody (12904-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP2W1 transfected HEK-293 cells.

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  WB analysis using 12904-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12904-1-AP (CYP2W1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12904-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12904-1-AP (CYP2W1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12904-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12904-1-AP (CYP2W1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 12904-1-AP

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 12904-1-AP (CYP2W1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human Colon cancer using 12904-1-AP

  Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 12904-1-AP (CYP2W1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12904-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP2W1 antibody (12904-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

12904-1-AP bindet in WB, IHC, IF/ICC, ELISA CYP2W1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYP2W1 fusion protein Ag3803
• Vollständiger Name: cytochrome P450, family 2, subfamily W, polypeptide 1
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 45-54 kDa
• GenBank-Zugangsnummer: BC025761
• Gene symbol: CYP2W1
• Gene ID (NCBI): 54905
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cytochrome P450s have been proposed to be of importance both in carcinogenesis, by activating precarcinogens, and as determinants of cancer chemotherapy, where they participate in activation or inactivation of anti-cancer drugs. CYP2W1 is exclusively expressed in transformed tissue in the adult human, mainly in colon tumors. It is a potential drug target in cancer therapy. CYP2W1 has a molecular weight of 52-54 kDa, and 30/45 kDa of potential isoforms.

Publikationen

Species	Application	Title
human	WB	Pharmacol Rep; Expression of CYP2S1 and CYP2W1 in breast cancer epithelial cells and modulation of their expression by synthetic methoxy stilbenes.; Authors - Hanna Szaefer