CYP3A4 Monoklonaler Antikörper

CYP3A4 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

2F11C4

Kat-Nr. : 67110-1-Ig

Synonyme

2F11C4, Cholesterol 25-hydroxylase, CP33, CP34, CYP3A

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  WB analysis of mouse liver using 67110-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67110-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 67110-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig gall bladder using 67110-1-Ig

  pig gall bladder tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 67110-1-Ig

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 67110-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 67110-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67110-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67110-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 67110-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse liver using 67110-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of rat liver using 67110-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 67110-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Mauslungengewebe, Hausschwein-Lebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 3 publications below

Produktinformation

67110-1-Ig bindet in WB, IHC, IF/ICC, IF-P, ELISA CYP3A4 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CYP3A4 fusion protein Ag13074
• Vollständiger Name: cytochrome P450, family 3, subfamily A, polypeptide 4
• Berechnetes Molekulargewicht: 503 aa, 57 kDa
• Beobachtetes Molekulargewicht: 51-57 kDa
• GenBank-Zugangsnummer: BC101631
• Gene symbol: CYP3A4
• Gene ID (NCBI): 1576
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Biomaterials; Hepatic endoplasmic reticulum-derived nanodiscs for broad-spectrum drug detoxification; Authors - Lei Sun
human	IF	Biomed Rep; Phenotypical, functional and transcriptomic comparison of two modified methods of hepatocyte differentiation from human induced pluripotent stem cells.; Authors - Rong Li
human	IF	Methods Mol Biol; Homogeneous Differentiation of Functional Hepatocytes from Human Induced Pluripotent Stem Cells.; Authors - Rong Li
human	IF	Acta Biomater; Hepar-on-a-sensor-platform with hybridization chain reaction amplification strategy to intuitively monitor the hepatoxicity of natural compounds; Authors - Wenhui Qian