CYP46A1 Polyklonaler Antikörper

CYP46A1 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

Kat-Nr. : 12486-1-AP

Synonyme

CP46, Cholesterol 24S-hydroxylase, Cholesterol 24-monooxygenase, Cholesterol 24-hydroxylase, Cholesterol 24 hydroxylase

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  WB analysis using 12486-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12486-1-AP (CYP46A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 12486-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12486-1-AP (CYP46A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12486-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12486-1-AP (CYP46A1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12486-1-AP

  IP result of anti-CYP46A1 (IP:12486-1-AP, 4ug; Detection:12486-1-AP 1:500) with mouse brain tissue lysate 3200ug.

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  IHC staining of mouse brain using 12486-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12486-1-AP (CYP46A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12486-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12486-1-AP (CYP46A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12486-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12486-1-AP (CYP46A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12486-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12486-1-AP (CYP46A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12486-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12486-1-AP (CYP46A1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 12486-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12486-1-AP (CYP46A1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse brain using 12486-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CYP46A1 antibody (12486-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 4 publications below

Produktinformation

12486-1-AP bindet in WB, IHC, IF-P, IP, ELISA CYP46A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYP46A1 fusion protein Ag3116
• Vollständiger Name: cytochrome P450, family 46, subfamily A, polypeptide 1
• Berechnetes Molekulargewicht: 500 aa, 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC022539
• Gene symbol: CYP46A1
• Gene ID (NCBI): 10858
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cytochrome P450 46A1 (CYP46A1), also named as CYP46, P450 46A1, CH24H and Cholesterol 24-hydroxylase, belongs to the cytochrome P450 family. CYP46A1 is a central nervous system-specific enzyme, and can freely cross the blood-brain barrier and be degraded in the liver. It is involved in the turnover of cholesterol. It converts cholesterol into 24S-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol. CYP46A1 is significantly upregulated in hepatic lymphomyeloid cells compared with ESCs.(PMID:20039312)

Publikationen

Species	Application	Title
human,mouse	WB,IHC	Cell Rep Med; Cholesterol 24-hydroxylase at the choroid plexus contributes to brain immune homeostasis; Authors - Afroditi Tsitsou-Kampeli
mouse	IF	Nat Commun; ATAD3A oligomerization promotes neuropathology and cognitive deficits in Alzheimer's disease models.; Authors - Yuanyuan Zhao
mouse	WB	EMBO Rep; NPC1 enables cholesterol mobilization during long-term potentiation that can be restored in Niemann-Pick disease type C by CYP46A1 activation.; Authors - Daniel N Mitroi
mouse	WB	Oncogene; Isocitrate dehydrogenase 1 mutation enhances 24(S)-hydroxycholesterol production and alters cholesterol homeostasis in glioma.; Authors - Risheng Yang
human	WB	Neurobiol Aging; H63D mutation in hemochromatosis alters cholesterol metabolism and induces memory impairment.; Authors - Fatima Ali-Rahmani
rat	IF	Sci Rep; 24(S)-Hydroxycholesterol protects the ex vivo rat retina from injury by elevated hydrostatic pressure.; Authors - Makoto Ishikawa