CYP46A1 Monoklonaler Antikörper

CYP46A1 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E10D3

Kat-Nr. : 68576-1-PBS

Synonyme

CH24H, Cholesterol 24 hydroxylase, CP46, CYP46, CYP46A1, Cytochrome P450 46A1

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  WB analysis using 68576-1-Ig (same clone as 68576-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68576-1-Ig (CYP46A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68576-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68576-1-Ig (same clone as 68576-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68576-1-Ig (CYP46A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68576-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68576-1-Ig (same clone as 68576-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68576-1-Ig (CYP46A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68576-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68576-1-PBS bindet in WB, IHC, Indirect ELISA CYP46A1 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CYP46A1 fusion protein Ag16786
• Vollständiger Name: cytochrome P450, family 46, subfamily A, polypeptide 1
• Berechnetes Molekulargewicht: 500 aa, 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC022539
• Gene symbol: CYP46A1
• Gene ID (NCBI): 10858
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cytochrome P450 46A1 (CYP46A1), also named as CYP46, P450 46A1, CH24H and Cholesterol 24-hydroxylase, belongs to the cytochrome P450 family. CYP46A1 is a central nervous system-specific enzyme, and can freely cross the blood-brain barrier and be degraded in the liver. It is involved in the turnover of cholesterol. It converts cholesterol into 24S-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol. CYP46A1 is significantly upregulated in hepatic lymphomyeloid cells compared with ESCs.(PMID:20039312)