CYP4F2-Specific Rekombinanter Antikörper

CYP4F2-Specific Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242307B10

Kat-Nr. : 85320-1-PBS

Synonyme

CYP4F2, 20-HETE synthase, 20-hydroxyeicosatetraenoic acid synthase, 242307B10, Arachidonic acid omega-hydroxylase

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  WB analysis using 85320-1-RR (same clone as 85320-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85320-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85320-1-RR (same clone as 85320-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85320-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 85320-1-RR (same clone as 85320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85320-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 85320-1-RR (same clone as 85320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85320-1-PBS in a different storage buffer formulation.

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  IHC staining of Human Hepatocellular Cancer using 85320-1-RR (same clone as 85320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85320-1-PBS in a different storage buffer formulation.

• 

  IHC staining of Human Hepatocellular Cancer using 85320-1-RR (same clone as 85320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85320-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85320-1-PBS bindet in WB, IHC, Indirect ELISA CYP4F2-Specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cytochrome P450, family 4, subfamily F, polypeptide 2
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 50-53 kDa
• GenBank-Zugangsnummer: NM_001082
• Gene symbol: CYP4F2
• Gene ID (NCBI): 8529
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYP4F2, also naemed as CPF2, belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, CYP4F2 is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. This antibody is specific to CYP4F2 with an observed molecular weight of 50-53 kDa (PMID: 17341693, PMID: 37121548).