CYP51A1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CYP51A1 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Rezensionen (1)

Kat-Nr. : 13431-1-AP

Synonyme

CP51, CYP51, CYP51A1, CYPL1, CYPLI, Cytochrome P450 14DM, Cytochrome P450 51A1, Cytochrome P45014DM, Cytochrome P450LI, LDM, P450 14DM, P450L1, Sterol 14 alpha demethylase

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Galerie der Validierungsdaten

• 

  WB analysis using 13431-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13431-1-AP (CYP51A1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 13431-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13431-1-AP (CYP51A1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 13431-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 13431-1-AP (CYP51A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 13431-1-AP

  IP Result of anti-CYP51A1 (IP:13431-1-AP, 4ug; Detection:13431-1-AP 1:1000) with mouse testis tissue lysate 2800ug.

• 

  IP experiment of mouse heart using 13431-1-AP

  IP result of anti-CYP51A1 (IP:13431-1-AP, 4ug; Detection:13431-1-AP 1:2000) with mouse heart tissue lysate 2200 ug.

• 

  IF Staining of PC-3 using 13431-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 13431-1-AP (CYP51A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HuH-7-Zellen, Maushodengewebe, Rattenlebergewebe
• Erfolgreiche IP: Maushodengewebe, Mausherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 21 publications below

Produktinformation

13431-1-AP bindet in WB, IP, ELISA CYP51A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYP51A1 fusion protein Ag4235
• Vollständiger Name: cytochrome P450, family 51, subfamily A, polypeptide 1
• Berechnetes Molekulargewicht: 509 aa, 57 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC032322
• Gene symbol: CYP51A1
• Gene ID (NCBI): 1595
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Pregnane X receptor agonist nomilin extends lifespan and healthspan in preclinical models through detoxification functions; Authors - Shengjie Fan
human	WB	Mol Cell; Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex; Authors - Michael L van de Weijer
human	WB	Mol Cell; Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.; Authors - Michael L van de Weijer
mouse	WB	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
human,mouse	WB	Nat Commun .; MMAB promotes negative feedback control of cholesterol homeostasis; Authors - Leigh Goedeke
human	WB	Protein Cell; POST1/C12ORF49 regulates the SREBP pathway by promoting site-1 protease maturation.; Authors - Jian Xiao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (12-01-2020)

Clear band with clean background

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse liver

CYP51A1 Antibody Western Blot validation (1:2000 dilution) in Mouse liver (Cat no:13431-1-AP)

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