CYP51A1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CYP51A1 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(29)

Rezensionen (1)

Kat-Nr. : 13431-1-AP

Synonyme

Cytochrome P450 51A1, Cytochrome P450 14DM, CYPLI, CYPL1, CYP51

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  WB analysis using 13431-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13431-1-AP (CYP51A1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 13431-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 13431-1-AP (CYP51A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 13431-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13431-1-AP (CYP51A1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 13431-1-AP

  IP result of anti-CYP51A1 (IP:13431-1-AP, 4ug; Detection:13431-1-AP 1:1000) with mouse testis tissue lysate 2800ug.

• 

  IP experiment of mouse heart using 13431-1-AP

  IP result of anti-CYP51A1 (IP:13431-1-AP, 4ug; Detection:13431-1-AP 1:2000) with mouse heart tissue lysate 2200 ug.

• 

  IF Staining of PC-3 using 13431-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 13431-1-AP (CYP51A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HuH-7-Zellen, Maushodengewebe, Rattenlebergewebe
• Erfolgreiche IP: Maushodengewebe, Mausherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 28 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

13431-1-AP bindet in WB, IHC, IF, IP, ELISA CYP51A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYP51A1 fusion protein Ag4235
• Vollständiger Name: cytochrome P450, family 51, subfamily A, polypeptide 1
• Berechnetes Molekulargewicht: 509 aa, 57 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC032322
• Gene symbol: CYP51A1
• Gene ID (NCBI): 1595
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lanosterol 14-alpha demethylase (cytochrome P450(51), CYP51A1) belongs to the evolutionarily conserved family of cytochrome P450 and catalyzes one of the key steps in cholesterol biosynthesis. CYP51A1 protein is targeted for degradation when exposed to nitric oxide generated under inflammatory conditions by NOS2 or released from NO donor compounds (PMID: 28830911). CYP51A1 is a prognostic marker in certain cancers, including Cervical squamous cell carcinoma, Head and neck squamous cell carcinoma, Kidney chromophobe, and Kidney renal clear cell carcinoma (PMID: 24711211).

Publikationen

Species	Application	Title
human	WB	Nature; 7-Dehydrocholesterol dictates ferroptosis sensitivity; Authors - Yaxu Li
mouse	WB	Nat Commun; Pregnane X receptor agonist nomilin extends lifespan and healthspan in preclinical models through detoxification functions; Authors - Shengjie Fan
human	WB	Mol Cell; Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex; Authors - Michael L van de Weijer
human	WB	Mol Cell; Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.; Authors - Michael L van de Weijer
mouse	WB	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
human,mouse	WB	Nat Commun .; MMAB promotes negative feedback control of cholesterol homeostasis; Authors - Leigh Goedeke

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (12-01-2020)

Clear band with clean background

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse liver

CYP51A1 Antibody Western Blot validation (1:2000 dilution) in Mouse liver (Cat no:13431-1-AP)