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CYP51A1 Rekombinanter Antikörper

CYP51A1 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242166G9

Kat-Nr. : 84705-4-PBS

Synonyme

242166G9, CP51, CYP51, CYPL1, CYPLI

Filter:
  • Western Blot (WB) analysis of various lysates using CYP51A1 Recombinant antibody (84705-4-RR)

    WB analysis using 84705-4-RR (same clone as 84705-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84705-4-RR (CYP51A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84705-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CYP51A1 Recombinant antibody (84705-4-RR)

    IHC staining of mouse testis using 84705-4-RR (same clone as 84705-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84705-4-RR (CYP51A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84705-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84705-4-RR

    Affinity and Kinetic Characterization of 84705-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84705-4-RR against Human CYP51A1 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

84705-4-PBS bindet in WB, IHC, Indirect ELISA CYP51A1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CYP51A1 fusion protein Ag4235
Vollständiger Name cytochrome P450, family 51, subfamily A, polypeptide 1
Berechnetes Molekulargewicht 509 aa, 57 kDa
Beobachtetes Molekulargewicht56 kDa
GenBank-ZugangsnummerBC032322
Gene symbol CYP51A1
Gene ID (NCBI) 1595
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lanosterol 14-alpha demethylase (cytochrome P450(51), CYP51A1) belongs to the evolutionarily conserved family of cytochrome P450 and catalyzes one of the key steps in cholesterol biosynthesis. CYP51A1 protein is targeted for degradation when exposed to nitric oxide generated under inflammatory conditions by NOS2 or released from NO donor compounds (PMID: 28830911). CYP51A1 is a prognostic marker in certain cancers, including Cervical squamous cell carcinoma, Head and neck squamous cell carcinoma, Kidney chromophobe, and Kidney renal clear cell carcinoma (PMID: 24711211).