CYP7A1 Polyklonaler Antikörper

CYP7A1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(22)

Kat-Nr. : 18054-1-AP

Synonyme

Cytochrome P450 7A1, CYPVII, CYP7, CP7A, Cholesterol 7-alpha-monooxygenase

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  WB analysis using 18054-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18054-1-AP (CYP7A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 18054-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18054-1-AP (CYP7A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 18054-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18054-1-AP (CYP7A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 18054-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18054-1-AP (CYP7A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18054-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18054-1-AP (CYP7A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 18054-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP7A1 antibody (18054-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HuH-7-Zellen, L02-Zellen, SMMC-7721-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 19 publications below
• IHC: See 4 publications below

Produktinformation

18054-1-AP bindet in WB, IHC, IF/ICC, ELISA CYP7A1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, hamster, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYP7A1 fusion protein Ag12572
• Vollständiger Name: cytochrome P450, family 7, subfamily A, polypeptide 1
• Berechnetes Molekulargewicht: 504 aa, 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC101777
• Gene symbol: CYP7A1
• Gene ID (NCBI): 1581
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYP7A1 (Cytochrome P450 Family 7 Subfamily A Member 1), is a cholesterol 7alpha-hydroxylase, catalyzing the first and rate-limiting step in the neutral or classic pathway for bile acid biosynthesis. CYP7A1 is a postulated gene modifier of colorectal cancer risk and target for the therapeutic bile acid, ursodeoxycholic acid (UDCA). The CYP7A1 enzyme controls the rate-limiting step of the catabolism of cholesterol into bile acids in the liver.

Publikationen

Species	Application	Title
mouse	WB	Cell Mol Gastroenterol Hepatol; Endoplasmic Reticulum Stress Regulates Hepatic Bile Acid Metabolism in Mice.; Authors - Anne S Henkel
human	WB	Cell Mol Gastroenterol Hepatol; A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.; Authors - Dayan Sun
mouse	IHC	Ecotoxicol Environ Saf; Triclosan exposure causes abnormal bile acid metabolism through IL-1β-NF-κB-Fxr signaling pathway; Authors - Jing Liu
mouse	WB	J Ethnopharmacol; Multi-omics and chemical profiling approaches to understand the material foundation and pharmacological mechanism of Sophorae Tonkinensis Radix et Rhizome-induced liver injury in mice; Authors - Si-Wei Rao
MOUSE	WB	J Lipid Res; Hepatic Deletion of X-box Binding Protein 1 Impairs Bile Acid Metabolism in Mice.; Authors - Xiaoying Liu
mouse	WB	Toxicol Sci; Hepatic, metabolic and toxicity evaluation of repeated oral administration of SnS2 nanoflowers in mice.; Authors - Disi Bai