CYP7B1 Polyklonaler Antikörper

CYP7B1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(30)

Kat-Nr. : 24889-1-AP

Synonyme

Cytochrome P450 7B1, CP7B, CBAS3, 3-hydroxysteroid 7-alpha hydroxylase, 25/26-hydroxycholesterol 7-alpha-hydroxylase

---

• 

  WB analysis of A431 using 24889-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 24889-1-AP (CYP7B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 24889-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24889-1-AP (CYP7B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 24889-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24889-1-AP (CYP7B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse liver using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human brain using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24889-1-AP (CYP7B1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24889-1-AP (CYP7B1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 24889-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CYP7B1 antibody (24889-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 24889-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CYP7B1 antibody (24889-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  IF Staining of HEK-293 using 24889-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CYP7B1 antibody (24889-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Hirngewebe, humanes Lebergewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 29 publications below
• IHC: See 2 publications below

Produktinformation

24889-1-AP bindet in WB, IHC, IF/ICC, ELISA CYP7B1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYP7B1 fusion protein Ag19374
• Vollständiger Name: cytochrome P450, family 7, subfamily B, polypeptide 1
• Berechnetes Molekulargewicht: 506 aa, 58 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC136574
• Gene symbol: CYP7B1
• Gene ID (NCBI): 9420
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYP7B1(Cytochrome P450 7B1) is also named as 25-hydroxycholesterol 7-alpha-hydroxylase and belongs to the cytochrome P450 family. It catalyzes the first and rate-limiting step in the coonversion of cholesterol to bile acids and CYP7B1-mediated catalysis may play a role for control of the cellular levels of androgens, not only of estrogens.

Publikationen

Species	Application	Title
human,mouse,rat	WB	Free Radic Biol Med; ALDH2 deficiency exacerbates MCD-diet induced MASLD by modulating bile acid metabolism; Authors - Jun Luo
mouse	WB	Cell Physiol Biochem; Ganoderma Lucidum Polysaccharide Peptide Alleviates Hepatoteatosis via Modulating Bile Acid Metabolism Dependent on FXR-SHP/FGF.; Authors - Dandan Zhong
mouse	WB	Mol Nutr Food Res; The Effect of Coenzyme Q10 Supplementation on Bile Acid Metabolism: Insights from Network Pharmacology, Molecular Docking, and Experimental Validation; Authors - Mengcheng Jin
mouse	WB	J Nutr Biochem; Deficiency of coiled-coil domain containing 80 increases plasma cholesterol by decreasing fecal sterols excretion in hypercholesterolemic mice.; Authors - Wenyun Li
mouse	WB	Biochem Pharmacol; Association between the perturbation of bile acid homeostasis and valproic acid-induced hepatotoxicity.; Authors - Yanan Chen
mouse	WB	J Lipid Res; Insulin resistance dysregulates CYP7B1 leading to oxysterol accumulation: a pathway for NAFL to NASH transition.; Authors - Genta Kakiyama