CYP7B1 Polyklonaler Antikörper

CYP7B1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24889-1-PBS

Synonyme

Cytochrome P450 7B1, CP7B, CBAS3, 3-hydroxysteroid 7-alpha hydroxylase, 25/26-hydroxycholesterol 7-alpha-hydroxylase

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  WB analysis of A431 using 24889-1-AP (same clone as 24889-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 24889-1-AP (CYP7B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 24889-1-AP (same clone as 24889-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24889-1-AP (CYP7B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 24889-1-AP (same clone as 24889-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24889-1-AP (CYP7B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24889-1-AP (CYP7B1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24889-1-AP (CYP7B1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 24889-1-AP (same clone as 24889-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CYP7B1 antibody (24889-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 24889-1-AP (same clone as 24889-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CYP7B1 antibody (24889-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 24889-1-AP (same clone as 24889-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CYP7B1 antibody (24889-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

24889-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CYP7B1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYP7B1 fusion protein Ag19374
• Vollständiger Name: cytochrome P450, family 7, subfamily B, polypeptide 1
• Berechnetes Molekulargewicht: 506 aa, 58 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC136574
• Gene symbol: CYP7B1
• Gene ID (NCBI): 9420
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYP7B1(Cytochrome P450 7B1) is also named as 25-hydroxycholesterol 7-alpha-hydroxylase and belongs to the cytochrome P450 family. It catalyzes the first and rate-limiting step in the coonversion of cholesterol to bile acids and CYP7B1-mediated catalysis may play a role for control of the cellular levels of androgens, not only of estrogens.