MT-CYB Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MT-CYB Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(115)

Rezensionen (3)

Kat-Nr. : 55090-1-AP

Synonyme

CYTB, COB, Complex III subunit 3, Complex III subunit III, MT CYB

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• 

  WB analysis using 55090-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55090-1-AP (CYTB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, MDA-MB-231-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 112 publications below
• IF: See 5 publications below

Produktinformation

55090-1-AP bindet in WB, IF, ELISA MT-CYB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cytochrome b
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: YP_003024038
• Gene symbol: CYTB
• Gene ID (NCBI): 4519
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYTB, also named as COB and MTCYB, is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. It's mitochondrion membrane protein with MW 43 kDa.

Publikationen

Species	Application	Title
human	WB	Cell Metab; Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.; Authors - Yi-Ping Wang
human	WB	Mol Cell; Hepatic micropeptide modulates mitochondrial RNA processing machinery in hepatocellular carcinoma; Authors - Linyu Zhu
human	WB	Nucleic Acids Res; Selection of initiator tRNA and start codon by mammalian mitochondrial initiation factor 3 in leaderless mRNA translation; Authors - Muhoon Lee
human	WB	Cell Rep Med; End binding-3 inhibitor activates regenerative program in age-related macular degeneration; Authors - Quinn Lee
human	WB	Nat Commun; RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis.; Authors - Uwe Richter
mouse	WB	Nat Commun; Mitofusin 1 and 2 regulation of mitochondrial DNA content is a critical determinant of glucose homeostasis.; Authors - Vaibhav Sidarala

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Baptiste (Verified Customer) (05-23-2025)

Working well but have a light unspecific band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Lymphoblastoid

MT-CYB Antibody Western Blot validation (1:1000 dilution) in Lymphoblastoid (Cat no:55090-1-AP)

FH

Yan (Verified Customer) (10-21-2024)

Worked fine in WB assay. Perhaps I should've used more concentrated.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human primary fibroblasts

MT-CYB Antibody Western Blot validation (1:1000 dilution) in Human primary fibroblasts (Cat no:55090-1-AP)

FH

Mi (Verified Customer) (02-10-2023)

Works very well in differentiated human white adipocyte cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: adipocytes