Cytohesin 2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Cytohesin 2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 10405-1-AP

Synonyme

ARF exchange factor, ARNO, CTS18, CTS18.1, CYTH2, cytohesin 2, Protein ARNO, PSCD2, PSCD2L, SEC7L, Sec7p L, Sec7p like

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Galerie der Validierungsdaten

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  WB analysis of mouse heart using 10405-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10405-1-AP (Cytohesin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10405-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10405-1-AP (Cytohesin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 10405-1-AP

  IP Result of anti-Cytohesin 2 (IP:10405-1-AP, 4ug; Detection:10405-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of human colon using 10405-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10405-1-AP (Cytohesin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10405-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10405-1-AP (Cytohesin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 10405-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10405-1-AP (Cytohesin 2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 10405-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10405-1-AP (Cytohesin 2 antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

10405-1-AP bindet in WB, IP, IHC, IF, ELISA Cytohesin 2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cytohesin 2 fusion protein Ag0687
• Vollständiger Name: cytohesin 2
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC004361
• Gene symbol: CYTH2
• Gene ID (NCBI): 9266
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pleckstrin homology, Sec7 and coiled/coil domains 2 (PSCD2) is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. PSCD2 exhibits GEP activity in vitro with small G proteins ARF1, ARF3, and ARF6.

Publikationen

Species	Application	Title
rat	WB, IF	J Neurosci; Inhibition of Cytohesins Protects against Genetic Models of Motor Neuron Disease.; Authors - Jinbin Zhai
mouse	WB,IHC	J Biol Chem; The synaptic scaffolding protein CNKSR2 interacts with CYTH2 to mediate hippocampal granule cell development.; Authors - Hidenori Ito ; KD Validated
human	WB	Oncol Rep; Cytohesin-2 as a novel prognostic marker for hepatocellular carcinoma.; Authors - Xu Kedong K
rat	WB,IP	Open Biol; ARNO is recruited by the neuronal adaptor FE65 to potentiate ARF6-mediated neurite outgrowth; Authors - Yuqi Zhai