CYTSA Polyklonaler Antikörper

CYTSA Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25390-1-PBS

Synonyme

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  WB analysis of NIH/3T3 using 25390-1-AP (same clone as 25390-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25390-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 25390-1-AP (same clone as 25390-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25390-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 25390-1-AP (same clone as 25390-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25390-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Neuro-2a using 25390-1-AP (same clone as 25390-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25390-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 25390-1-AP (same clone as 25390-1-PBS)

  IP result of anti-CYTSA (IP:25390-1-AP, 4ug; Detection:25390-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 25390-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 25390-1-AP (same clone as 25390-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25390-1-AP (CYTSA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25390-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 25390-1-AP (same clone as 25390-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25390-1-AP (CYTSA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25390-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

25390-1-PBS bindet in WB, IHC, IP, Indirect ELISA CYTSA und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYTSA fusion protein Ag21896
• Vollständiger Name: cytospin A
• Berechnetes Molekulargewicht: 1117 aa, 125 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC136471
• Gene symbol: CYTSA
• Gene ID (NCBI): 23384
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYTSA, also named as SPECC1L or KIAA0376, is a 1117 amino acid protein, which contains one CH domain and belongs to the cytospin-A family. CYTSA localizes in the cytoplasm and is involved in cytokinesis and spindle organization. CYTSA may play a role in actin cytoskeleton organization and microtubule stabilization, hence it is required for proper cell adhesion and migration.