Cadherin-13 Polyklonaler Antikörper

Cadherin-13 Polyklonal Antikörper für WB, IHC, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12618-1-PBS

Synonyme

CDH13, Cadherin 13, CDHH, H cadherin, H-cadherin

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  WB analysis of mouse heart using 12618-1-AP (same clone as 12618-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12618-1-AP (Cadherin-13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12618-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 12618-1-AP (same clone as 12618-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 12618-1-AP (Cadherin-13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12618-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 12618-1-AP (same clone as 12618-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 12618-1-AP (Cadherin-13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12618-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse heart using 12618-1-AP (same clone as 12618-1-PBS)

  IP result of anti-Cadherin-13 (IP:12618-1-AP, 4ug; Detection:12618-1-AP 1:700) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 12618-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 12618-1-AP (same clone as 12618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12618-1-AP (Cadherin-13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12618-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 12618-1-AP (same clone as 12618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12618-1-AP (Cadherin-13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12618-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 12618-1-AP (same clone as 12618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12618-1-AP (Cadherin-13 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12618-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 12618-1-AP (same clone as 12618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12618-1-AP (Cadherin-13 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12618-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 12618-1-AP (same clone as 12618-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Cadherin-13 antibody (12618-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12618-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 12618-1-AP (same clone as 12618-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Cadherin-13 antibody (12618-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12618-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12618-1-PBS bindet in WB, IHC, IF-P, IP, Indirect ELISA Cadherin-13 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cadherin-13 fusion protein Ag3295
• Vollständiger Name: cadherin 13, H-cadherin (heart)
• Berechnetes Molekulargewicht: 713 aa, 78 kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: BC030653
• Gene symbol: Cadherin-13
• Gene ID (NCBI): 1012
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Cadherin-13 (CDH13, also known as H-cadherin or T-cadherin) is an atypical member of cadherin family, lacking transmembrane and cytosolic domains and anchored to the exterior surface of the plasma membrane via a GPI anchor. CDH13 is thought to affect cellular behavior largely through its signaling properties. It is highly expressed in heart, and in the CNS, CDH13 is expressed in cerebral cortex, medulla, hippocampus, amygdala, thalamus and substantia nigra. It is often down-regulated in cancerous cells. The CDH13 cDNA encodes a 713-amino acid protein, which has a calculated molecular weight of 78 kDa. Studies have detected variable bands on Western blots, including 105 kDa (mature form), 130 kDa (precursor), and 45 kDa, which may be a post-translational processing variant of CDH13 (PMID: 19399994).