Cadherin-17 Monoklonaler Antikörper

Cadherin-17 Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3B10A9

Kat-Nr. : 60351-1-PBS

Synonyme

CDH17, 3B10A9, Cadherin 17, CDH16, HPT 1

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  WB analysis of human colon using 60351-1-Ig (same clone as 60351-1-PBS)

  Untreated and PNGase F-treated lysates of human colon tissue were subjected to SDS PAGE followed by western blot with 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

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  WB analysis of pig colon using 60351-1-Ig (same clone as 60351-1-PBS)

  pig colon tissue were subjected to SDS PAGE followed by western blot with 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

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  WB analysis of human colon using 60351-1-Ig (same clone as 60351-1-PBS)

  human colon tissue were subjected to SDS PAGE followed by western blot with 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60351-1-Ig (same clone as 60351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded colon cancer slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60351-1-Ig (same clone as 60351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded colon cancer slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60351-1-Ig (same clone as 60351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60351-1-Ig (same clone as 60351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 60351-1-Ig (same clone as 60351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 60351-1-Ig (same clone as 60351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60351-1-Ig (same clone as 60351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded colon cancer slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60351-1-Ig (same clone as 60351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60351-1-Ig (same clone as 60351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 60351-1-Ig (same clone as 60351-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Cadherin-17 antibody (60351-1-Ig, Clone: 3B10A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 60351-1-Ig (same clone as 60351-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Cadherin-17 antibody (60351-1-Ig, Clone: 3B10A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60351-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA Cadherin-17 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cadherin-17 fusion protein Ag19488
• Vollständiger Name: cadherin 17, LI cadherin (liver-intestine)
• Berechnetes Molekulargewicht: 832 aa, 92 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC113464
• Gene symbol: Cadherin-17
• Gene ID (NCBI): 1015
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.