Calmegin Polyklonaler Antikörper

Calmegin Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12629-1-PBS

Synonyme

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  WB analysis of mouse testis using 12629-1-AP (same clone as 12629-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12629-1-AP (Calmegin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 12629-1-AP (same clone as 12629-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12629-1-AP (Calmegin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human lung using 12629-1-AP (same clone as 12629-1-PBS)

  human lung tissue were subjected to SDS PAGE followed by western blot with 12629-1-AP (Calmegin antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 12629-1-AP (same clone as 12629-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12629-1-AP (Calmegin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 12629-1-AP (same clone as 12629-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12629-1-AP (Calmegin antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse testis using 12629-1-AP (same clone as 12629-1-PBS)

  IP result of anti-Calmegin (IP:12629-1-AP, 4ug; Detection:12629-1-AP 1:1000) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 12629-1-AP (same clone as 12629-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12629-1-AP (Calmegin antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 12629-1-AP (same clone as 12629-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12629-1-AP (Calmegin antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12629-1-PBS bindet in WB, IHC, IP, Indirect ELISA Calmegin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Calmegin fusion protein Ag3322
• Vollständiger Name: calmegin
• Berechnetes Molekulargewicht: 610 aa, 70 kDa
• Beobachtetes Molekulargewicht: 93 kDa
• GenBank-Zugangsnummer: BC028357
• Gene symbol: Calmegin
• Gene ID (NCBI): 1047
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calmegin, belonging to the calreticulin family, is a testis-specific molecular chaperon of the endoplasmic reticulum playing a key role in spermatogenesis. Western blot analysis of mouse testis showed a single band with an apparent molecular mass of 93 kDa.