Calpain 11 Polyklonaler Antikörper

Calpain 11 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13675-1-PBS

Synonyme

EC:3.4.22.-, CAPN11, CANP 11, Calpain-11, calpain11

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  WB analysis of PC-3 using 13675-1-AP (same clone as 13675-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13675-1-AP (Calpain 11 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13675-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 13675-1-AP (same clone as 13675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13675-1-AP (Calpain 11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13675-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13675-1-AP (same clone as 13675-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Calpain 11 antibody (13675-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 13675-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13675-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Calpain 11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Calpain 11 fusion protein Ag4668
• Vollständiger Name: calpain 11
• Berechnetes Molekulargewicht: 702 aa, 81 kDa
• Beobachtetes Molekulargewicht: 81 kDa
• GenBank-Zugangsnummer: BC033733
• Gene symbol: Calpain 11
• Gene ID (NCBI): 11131
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calpains are a superfamily of related proteins, some of which have been shown to function as intracellular calcium-dependent cysteine proteases. Calpains consist of a variable large (80 kDa) subunit and an invariant small (30 kDa) subunit. The expression of calpain11 during spermatogenesis and its localization is spermatozoa suggest that it is involved in regulating calcium-dependent signal transduction events during meiosis and sperm functional processes(PMID:16541461). And the 110 kDa band protein may be regarded as a complex.