Calpain 3 Polyklonaler Antikörper

Calpain 3 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10415-1-PBS

Synonyme

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  WB analysis of mouse skeletal muscle using 10415-1-AP (same clone as 10415-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10415-1-AP (Calpain 3 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10415-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 10415-1-AP (same clone as 10415-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10415-1-AP (Calpain 3 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10415-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human urothelial carcinoma using 10415-1-AP (same clone as 10415-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10415-1-AP (Calpain 3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10415-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human urothelial carcinoma using 10415-1-AP (same clone as 10415-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10415-1-AP (Calpain 3 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10415-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10415-1-PBS bindet in WB, IHC, Indirect ELISA Calpain 3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Calpain 3 fusion protein Ag0671
• Vollständiger Name: calpain 3, (p94)
• Berechnetes Molekulargewicht: 94 kDa
• Beobachtetes Molekulargewicht: 90-105 kDa, 55-60 kDa, 30-35 kDa
• GenBank-Zugangsnummer: BC003521
• Gene symbol: CAPN3
• Gene ID (NCBI): 825
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calpain 3 (CAPN3), a major intracellular protease, is a muscle-specific member of the calpain large subunit family that specifically binds to titin. CAPN3 plays a role in the dysferlin protein complex and that disruption of CAPN3 function may affect muscle membrane repair and remodeling. CAPN3 has some isoforms with MW of 94, 84, 93, 36, 18 kDa. CAPN3 autolysis generates a small N-terminal fragment of 34 kDa and a large C-terminal fragment whose size ranges from 55 to 60 kDa during self-processing (PMID: 9794799, 14645524).

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