Calpain 7 Polyklonaler Antikörper

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• KD/KO Validated

Calpain 7 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 26985-1-AP

Synonyme

CAPN7, PalBH, EC:3.4.22.-, Calpain-7, CALPAIN7

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  WB analysis of mouse brain using 26985-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26985-1-AP (Calpain 7 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26985-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26985-1-AP (Calpain 7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26985-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26985-1-AP (Calpain 7 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 26985-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 26985-1-AP (Calpain 7 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Produktinformation

26985-1-AP bindet in WB, IHC, IF, ELISA Calpain 7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Calpain 7 fusion protein Ag25574
• Vollständiger Name: calpain 7
• Berechnetes Molekulargewicht: 93 kDa
• Beobachtetes Molekulargewicht: 92 kDa
• GenBank-Zugangsnummer: BC056202
• Gene symbol: Calpain 7
• Gene ID (NCBI): 23473
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain 7 (also known as PalBH) is a mammalian homologue of the Aspergillus, atypical calpain PalB. It is a unique intracellular cysteine protease that has a tandem repeat of microtubule interacting and trafficking (MIT) domains and lacks a penta-EF-hand domain.

Publikationen

Species	Application	Title
mouse	WB	EMBO Mol Med; CRTH2 promotes endoplasmic reticulum stress-induced cardiomyocyte apoptosis through m-calpain.; Authors - Shengkai Zuo
human	WB,IF	Elife; Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission; Authors - Dawn M Wenzel
human	WB,IF	Elife; The Calpain-7 protease functions together with the ESCRT-III protein IST1 within the midbody to regulate the timing and completion of abscission; Authors - Elliott L Paine ; KD Validated