Calponin Polyklonaler Antikörper

Calponin Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Kat-Nr. : 24855-1-AP

Synonyme

CNN1, CNN 1, Calponin-1, Calponin 1, Basic calponin

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  WB analysis of mouse stomach using 24855-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 24855-1-AP (Calponin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 24855-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 24855-1-AP (Calponin antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 24855-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24855-1-AP (Calponin Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 24855-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24855-1-AP (Calponin Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast hyperplasia using 24855-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 24855-1-AP (Calponin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 24855-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24855-1-AP (Calponin Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 24855-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24855-1-AP (Calponin Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse heart using 24855-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Calponin antibody (24855-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of C2C12 using 24855-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Calponin antibody (24855-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Magengewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Mammahyperplasie-Gewebe, humanes Herzgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausherzgewebe
• Erfolgreiche Detektion in IF/ICC: C2C12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 14 publications below

Produktinformation

24855-1-AP bindet in WB, IHC, IF/ICC, IF-P, ELISA Calponin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Calponin fusion protein Ag21384
• Vollständiger Name: calponin 1, basic, smooth muscle
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC022015
• Gene symbol: Calponin
• Gene ID (NCBI): 1264
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kDa protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the specific region of human CNN1, thus it does not cross-react with CNN2 or CNN3.

Publikationen

Species	Application	Title
human	WB	Bioact Mater; A TEMPOL and rapamycin loaded nanofiber-covered stent favors endothelialization and mitigates neointimal hyperplasia and local inflammation.; Authors - Rui Wang
human	WB	Mol Ther Nucleic Acids; Long Non-coding RNA PEBP1P2 Suppresses Proliferative VSMCs Phenotypic Switching and Proliferation in Atherosclerosis.; Authors - Xingqiang He
rat	WB	J Ginseng Res; Protective effect and mechanism of ginsenoside Rg2 on atherosclerosis; Authors - Qianqian Xue
mouse	WB	Sci Rep; Nicotine facilitates VSMC dysfunction through a miR-200b/RhoGDIA/cytoskeleton module.; Authors - Dongli Liang
human,rat	WB	Front Mol Biosci; Integrated Strategies of Diverse Feature Selection Methods Identify Aging-Based Reliable Gene Signatures for Ischemic Cardiomyopathy.; Authors - Huafeng Song
human	WB	J Mol Cell Cardiol; miR-564: A potential regulator of vascular smooth muscle cells and therapeutic target for aortic dissection.; Authors - Min Li