Calponin Monoklonaler Antikörper

Calponin Monoklonal Antikörper für WB, IHC, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A11E5

Kat-Nr. : 66540-1-PBS

Synonyme

Basic calponin, Calponin, Calponin 1, Calponin H1, smooth muscle, CNN1, Sm Calp, SMCC

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  WB analysis using 66540-1-Ig (same clone as 66540-1-PBS)

  Various tissue were subjected to SDS PAGE followed by western blot with 66540-1-Ig (Calponin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66540-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50017-1

  Sandwich ELISA standard curve ofMP50017-1, Calponin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66540-2-PBS. Detection antibody: HRP-conjugated 66540-1-PBS. Standard: Ag21384. Range: 0.313-20 ng/mL.

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  IHC staining of human myofibroblastoma using 66540-1-Ig (same clone as 66540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human myofibroblastoma tissue slide using 66540-1-Ig (Calponin antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66540-1-PBS in a different storage buffer formulation.

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  IHC staining of human myofibroblastoma using 66540-1-Ig (same clone as 66540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human myofibroblastoma tissue slide using 66540-1-Ig (Calponin antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66540-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66540-1-PBS bindet in WB, IHC, Sandwich ELISA, Indirect ELISA Calponin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Calponin fusion protein Ag21384
• Vollständiger Name: calponin 1, basic, smooth muscle
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 33-35 kDa
• GenBank-Zugangsnummer: BC022015
• Gene symbol: Calponin
• Gene ID (NCBI): 1264
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kD protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the specific region of human CNN1, thus it does not cross-react with CNN2 or CNN3.