Calretinin Monoklonaler Antikörper

Calretinin Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, IF-Fro, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, IF-Fro, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2D7A9

Kat-Nr. : 66496-1-PBS

Synonyme

CALB2, 29 kDa calbindin, 2D7A9, CAB29, CAL2

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  WB analysis using 66496-1-Ig (same clone as 66496-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat cerebellum using 66496-1-Ig (same clone as 66496-1-PBS)

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse cerebellum using 66496-1-Ig (same clone as 66496-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66496-1-Ig (same clone as 66496-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 66496-1-Ig (same clone as 66496-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66496-1-Ig (same clone as 66496-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-251 using 66496-1-Ig (same clone as 66496-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 66496-1-Ig (same clone as 66496-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cerebellum using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cerebellum using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat cerebellum using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat cerebellum tissue using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat cerebellum using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat cerebellum tissue using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse cerebellum using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse cerebellum tissue using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50295-3

  Cytometric bead array standard curve of MP50295-3, Calretinin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66496-6-PBS. Detection antibody: 66496-1-PBS. Standard:Ag2924. Range: 3.125-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66496-1-PBS bindet in WB, IHC, IF/ICC, IF-P, IF-Fro, Cytometric bead array, Indirect ELISA Calretinin und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Calretinin fusion protein Ag2924
• Vollständiger Name: calbindin 2
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC015484
• Gene symbol: Calretinin
• Gene ID (NCBI): 794
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.