Calretinin Rekombinanter Antikörper

Calretinin Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

2F20

Kat-Nr. : 82811-1-RR

Synonyme

CALB2, 29 kDa calbindin, 2F20, CAB29, CAL2

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  WB analysis using 82811-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82811-1-RR (Calretinin antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 82811-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82811-1-RR (Calretinin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 82811-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82811-1-RR (Calretinin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 82811-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82811-1-RR (Calretinin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of adult mouse cerebellum tissue using 82811-1-RR

  Immunofluorescent analysis of (4% PFA) fixed adult mouse cerebellum tissue using Calretinin antibody (82811-1-RR) at dilution of 1:500 and Alexa Fluor 594 AffiniPure Donkey Anti-Rabbit IgG (H+L). (Image produced by Watt Lab at McGill University)

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  IF Staining of SH-SY5Y using 82811-1-RR

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Calretinin antibody (82811-1-RR, Clone: 2F20 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

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  FC experiment of SH-SY5Y using 82811-1-RR

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Calretinin Recombinant antibody (82811-1-RR, Clone:2F20) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, fetales humanes Hirngewebe, Maushirngewebe, Hausschwein-Hirngewebe, Rattenhirngewebe, U2OS-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen
• Erfolgreiche Detektion in FC (Intra): SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5100
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82811-1-RR bindet in WB, IHC, IF/ICC, FC (Intra), ELISA Calretinin und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Calretinin fusion protein Ag2924
• Vollständiger Name: calbindin 2
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC015484
• Gene symbol: Calretinin
• Gene ID (NCBI): 794
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.