CPE Polyklonaler Antikörper

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• KD/KO Validated

CPE Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13710-1-PBS

Synonyme

Carboxypeptidase E, Carboxypeptidase H, CPH, EC:3.4.17.10, Prohormone-processing carboxypeptidase

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  WB analysis of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

  IP result of anti-Carboxypeptidase E (IP:13710-1-AP, 3ug; Detection:13710-1-AP 1:500) with mouse brain tissue lysate 3500ug. This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 13710-1-AP (same clone as 13710-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

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  IHC staining of human pituitary using 13710-1-AP (same clone as 13710-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

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  IHC staining of human pituitary using 13710-1-AP (same clone as 13710-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Carboxypeptidase E antibody (13710-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 13710-1-AP (same clone as 13710-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Carboxypeptidase E antibody (13710-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13710-1-PBS bindet in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA CPE und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CPE fusion protein Ag4647
• Vollständiger Name: carboxypeptidase E
• Berechnetes Molekulargewicht: 476 aa, 53 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC033866
• Gene symbol: Carboxypeptidase E
• Gene ID (NCBI): 1363
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CPE(Carboxypeptidase E) is also named as CPH.It belongs to the peptidase M14 family and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin.The deduced 476-amino acid human protein contains an N-terminal signal peptide, followed by a 'pro' sequence, a polyarginine stretch, and 2 potential N-glycosylation sites.