Cardiac Troponin I Monoklonaler Antikörper

Cardiac Troponin I Monoklonal Antikörper für WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

1D5D6

Kat-Nr. : 66376-1-PBS

Synonyme

cTnI, 1D5D6, TNNI3

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  WB analysis using 66376-1-Ig (same clone as 66376-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 66376-1-Ig (same clone as 66376-1-PBS)

  Human heart tissue and skeletal muscle tissue (negative control) were subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin I Antibody) at dilution of 1:20,000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

  Mouse heart tissue (6 ug/lane) was subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin I Antibody) at dilution of 1:5,000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 66376-1-Ig (same clone as 66376-1-PBS)

  Rat heart tissue were subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin Iantibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP51198-1

  Sandwich ELISA standard curve of MP51198-1, Human Cardiac Troponin I Monoclonal Matched Antibody Pair - PBS only. 66376-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag16758. 66376-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  Sample test of MP51198-1

  The mean Cardiac Troponin I concentration was determined to be 220.78 ng/mL in mouse heart tissue extract based on a 7.10 mg/mL extract load.

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  Sample test of MP51198-1

  Cytometric bead array in cell lysate using MP51198-1, Cardiac Troponin I Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66376-2-PBS. Detection antibody: 66376-1-PBS. Cell lysate: Rabbit muscle, Rabbit heart.

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  IHC staining of human heart using 66376-1-Ig (same clone as 66376-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:600 (under 10x lens). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66376-1-Ig (same clone as 66376-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:600 (under 40x lens). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Cardiac Troponin I antibody (66376-1-Ig, Clone: 1D5D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

  Immunofluorescent analysis of (4% PFA ) fixed mouse heart tissue using 66376-1-Ig(Cardiac Troponin I antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP51198-1

  Cytometric bead array standard curve of MP51198-1, Cardiac Troponin I Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66376-2-PBS. Detection antibody: 66376-1-PBS. Standard:Ag16758. Range: 12.5-400 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66376-1-PBS bindet in WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Cardiac Troponin I und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cardiac Troponin I fusion protein Ag16758
• Vollständiger Name: troponin I type 3 (cardiac)
• Berechnetes Molekulargewicht: 210 aa, 24 kDa
• Beobachtetes Molekulargewicht: 26-28 kDa
• GenBank-Zugangsnummer: BC096165
• Gene symbol: Cardiac Troponin I
• Gene ID (NCBI): 7137
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Troponin I (TnI) is the inhibitory subunit of troponin, the structural protein involved in the regulation of striated muscle contraction. Cardiac Troponin I (cTnI) is produced by cardiac muscle. Two other TnI isoforms (slow sTnI and fast sTnI) are produced by slow-twitch and fast-twitch skeletal muscles, respectively. cTnI has been considered as one of the most specific and sensitive markers of myocardial damage like acute myocardial infarction (AMI) for decades. Detection of cTnI in serum is widely accepted as a diagnostic tool in acute coronary syndromes. This antibody is specific to cTnI, but not cross-react with skeletal TnI.