Cardiac Troponin T Polyklonaler Antikörper

Cardiac Troponin T Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 26592-1-AP

Synonyme

Cardiac muscle troponin T, Cardiac Troponin T, CMH2, CMPD2, cTnT, RCM3, TNNT2, TnTC, troponin T type 2 (cardiac), Troponin T, cardiac muscle

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  WB analysis using 26592-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26592-1-AP (Cardiac Troponin T antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 26592-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26592-1-AP (Cardiac Troponin T antibody at dilution of 1:500 (under 10x lens).

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  IHC staining of human heart using 26592-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26592-1-AP (Cardiac Troponin T antibody at dilution of 1:500 (under 40x lens).

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  IHC staining of mouse heart using 26592-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26592-1-AP (Cardiac Troponin T antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 26592-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26592-1-AP (Cardiac Troponin T antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 26592-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 26592-1-AP (Cardiac Troponin T antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse heart using 26592-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Cardiac Troponin T antibody (26592-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 7 publications below

Produktinformation

26592-1-AP bindet in WB, IHC, IF-P, ELISA Cardiac Troponin T und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cardiac Troponin T fusion protein Ag24911
• Vollständiger Name: troponin T type 2 (cardiac)
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 34-40 kDa
• GenBank-Zugangsnummer: BC002653
• Gene symbol: Cardiac Troponin T
• Gene ID (NCBI): 7139
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IF	Biomaterials; Targeted immunomodulation therapy for cardiac repair by platelet membrane engineering extracellular vesicles via hitching peripheral monocytes.; Authors - Qiyu Li
human	IF	Free Radic Biol Med; SIRT3 promotes metabolic maturation of human iPSC-derived cardiomyocytes via OPA1-controlled mitochondrial dynamics; Authors - Rui Wang
mouse	IHC	Front Physiol; The physiological response during optogenetic-based cardiac pacing in awake freely moving mice; Authors - Jun Kaminosono
mouse	IF	Clin Exp Hypertens; Exploration and validation of signature genes and immune associations in septic cardiomyopathy; Authors - Zhengbo Zhao
human,mouse	IF	Signal Transduct Target Ther; Thiamine-modified metabolic reprogramming of human pluripotent stem cell-derived cardiomyocyte under space microgravity; Authors - Xinglong Han
mouse	IF	Nat Commun; Fibroblast-specific PRMT5 deficiency suppresses cardiac fibrosis and left ventricular dysfunction in male mice; Authors - Yasufumi Katanasaka