Cas9 Polyklonaler Antikörper

Cas9 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Bakterien und mehr (1)

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 26758-1-AP

Synonyme

dCas9, nCas9, Cas9 7A9, CRISPR, CRISPR/Cas9

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  WB analysis of HeLa using 26758-1-AP

  HeLa cells transfected with CRISPR-Cas9 were subjected to SDS PAGE followed by western blot with 26758-1-AP (Cas9 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 26758-1-AP

  A431 cells transfected with CRISPR-Cas9 were subjected to SDS PAGE followed by western blot with 26758-1-AP (Cas9 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 26758-1-AP

  IP result of anti-Cas9 (IP:26758-1-AP, 4ug; Detection:26758-1-AP 1:1000) with HeLa cells transfected with CRISPR-Cas9 600 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

26758-1-AP bindet in WB, IP, ELISA Cas9 und zeigt Reaktivität mit Bakterien

• Getestete Reaktivität: Bakterien
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cas9 fusion protein Ag25281
• Vollständiger Name: Cas9
• Berechnetes Molekulargewicht: 160 kDa
• GenBank-Zugangsnummer: NP_269215
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes. This antibody detects the sp Cas9.
This antibody can recognize Cas9 protein with point mutations, such as dCas9 and nCas9.

Publikationen

Species	Application	Title
	WB	Nature; Enhancer release and retargeting activates disease-susceptibility genes.; Authors - Soohwan Oh
human	WB	Adv Sci (Weinh); Enhancing Prime Editing Efficiency Through Modulation of Methylation on the Newly Synthesized DNA Strand and Prolonged Expression; Authors - Xiaosong Han
human	WB	Clin Epigenetics; E2F1 combined with LINC01004 super-enhancer to promote hepatocellular carcinoma cell proliferation and metastasis; Authors - Jingxuan Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kiara (Verified Customer) (07-28-2025)

This primary antibody is not validated for IF, but I gave it a shot anyways. As expected, it did not give a reliable signal.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: iPSC-derived cardiomyocytes

Cas9 Antibody Immunofluorescence validation (1:400 dilution) in iPSC-derived cardiomyocytes (Cat no:26758-1-AP)