Casein Kinase 1 Delta Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Casein Kinase 1 Delta Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14388-1-PBS

Synonyme

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  WB analysis using 14388-1-AP (same clone as 14388-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14388-1-AP (Casein Kinase 1 Delta antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14388-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 14388-1-AP (same clone as 14388-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14388-1-AP (Casein Kinase 1 delta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14388-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 14388-1-AP (same clone as 14388-1-PBS)

  IP result of anti-Casein Kinase 1 Delta (IP:14388-1-AP, 3ug; Detection:14388-1-AP 1:1000) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 14388-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 14388-1-AP (same clone as 14388-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14388-1-AP (Casein Kinase 1 Delta antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14388-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 14388-1-AP (same clone as 14388-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14388-1-AP (Casein Kinase 1 Delta antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14388-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14388-1-AP (same clone as 14388-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Casein Kinase 1 Delta antibody (14388-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14388-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U-251 using 14388-1-AP (same clone as 14388-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using Casein Kinase 1 Delta antibody (14388-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14388-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14388-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA Casein Kinase 1 Delta und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Casein Kinase 1 Delta fusion protein Ag5856
• Vollständiger Name: casein kinase 1, delta
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC003558
• Gene symbol: CK1 Delta
• Gene ID (NCBI): 1453
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSNK1D(casein kinase I isoform delta) is also named as HCKID, CKId and belongs to the casein kinase I subfamily. CKI is a ubiquitous enzyme which has been found in the cytosol, associated with membranes, and in the nucleus. It can phosphorylate a large number of cytosolic, membrane-associated, and nuclear proteins(PMID:8454611). CSNK1D is found to be associated generally with granulovacuolar bodies and tau-containing neurofibrillary tangles in AD, DS, PSP, PDC, PPND, and controls, and Pick bodies and ballooned neurons in PiD(PMID:10924763). It has 2 isoforms produced by alternative splicing.