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Caspase 1 Rekombinanter Antikörper

Caspase 1 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1O18

Kat-Nr. : 81482-1-PBS

Synonyme

CASP1, Caspase1, 1O18, CASP 1, CASP-1

Filter:
  • Western Blot (WB) analysis of various lysates using Caspase 1 Recombinant monoclonal antibody (81482-1-RR)

    WB analysis using 81482-1-RR (same clone as 81482-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81482-1-RR (Caspase 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81482-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81482-1-RR

    Affinity and Kinetic Characterization of 81482-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81482-1-RR against Human Caspase 1 were performed. The affinity constant is 305 nM.

Geprüfte Anwendungen

Produktinformation

81482-1-PBS bindet in WB, Indirect ELISA Caspase 1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Caspase 1 fusion protein Ag11143
Vollständiger Name caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
Berechnetes Molekulargewicht 404 aa, 45 kDa
Beobachtetes Molekulargewicht30 kDa, 35 kDa, 43-45 kDa
GenBank-ZugangsnummerBC062327
Gene symbol Caspase 1
Gene ID (NCBI) 834
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products (PMID:21804020). It has 5 isoforms produced by alternative splicing. 81482-1-RR can recognize the 45 kDa Pro-Caspase1 and the 30-35 kDa splicing isoforms or active dimer form of caspase 1.