Caspase 1 Rekombinanter Antikörper

Caspase 1 Rekombinant Antikörper für WB

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB

Konjugation

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

1O18

Kat-Nr. : CL750-81482

Synonyme

CASP1, Caspase1, Caspase-1, CASP-1, CASP 1

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  WB analysis using CL750-81482

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-81482 (Caspase 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, U-937-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL750-81482 bindet in WB Caspase 1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Caspase 1 fusion protein Ag11143
• Vollständiger Name: caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
• Berechnetes Molekulargewicht: 404 aa, 45 kDa
• Beobachtetes Molekulargewicht: 30 kDa, 35 kDa, 43-45 kDa
• GenBank-Zugangsnummer: BC062327
• Gene symbol: Caspase 1
• Gene ID (NCBI): 834
• Konjugation: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products (PMID:21804020). It has 5 isoforms produced by alternative splicing. 81482-1-RR can recognize the splicing isoforms of caspase 1.